Immunocytochemical methods and protocols /

Immunocytochemical methods and protocols /

Saved in:

Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, NJ : Humana Press, ©1999.
Description:	1 online resource (xii, 465 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; v. 115 Methods in molecular biology (Clifton, N.J.) ; v. 115.
Subject:	Immunocytochemistry -- Laboratory manuals. In situ hybridization -- Laboratory manuals. Immunohistochemistry -- methods. Immunohistochemistry -- standards. Immunocytochemistry. In situ hybridization. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11106761

Hidden Bibliographic Details
Other authors / contributors:	Javois, Lorette C.
ISBN:	9781592592135 1592592139 0585246181 9780585246185 1280830212 9781280830211 0896038130 9780896038134 0896035700 9780896035706
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.
Other form:	Print version: Immunocytochemical methods and protocols. 2nd ed. Totowa, NJ : Humana Press, ©1999 0896038130
Standard no.:	10.1385/1592592139.

Description
Summary:	Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.<br>
Physical Description:	1 online resource (xii, 465 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	9781592592135 1592592139 0585246181 9780585246185 1280830212 9781280830211 0896038130 9780896038134 0896035700 9780896035706