DNA repair protocols : prokaryotic systems /

DNA repair protocols : prokaryotic systems /

Saved in:
Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2000.
Description:1 online resource (x, 209 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 152
Methods in molecular biology (Clifton, N.J.) ; v. 152.
Subject:
DNA repair -- Laboratory manuals.
DNA Repair.
Prokoryotic Cells -- cytology
DNA repair.
Laboratory Manual.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11113059
Hidden Bibliographic Details
Other authors / contributors:Vaughan, Pat.
ISBN:1592590683
9781592590681
0585354332
9780585354330
089603643X
9780896036437
1280820594
9781280820595
9786610820597
6610820597
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.
Other form:Print version: DNA repair protocols. Totowa, N.J. : Humana Press, ©2000 089603643X
Standard no.:10.1385/1592590683.
Table of Contents:
  • pt. 1. Assays to detect and quantitate DNA repair activity
  • Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase
  • Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1)
  • Detection and quantitation of uracil DNA glycosylase activity
  • Detection of DNA deoxyribophosphodiesterase activity
  • O⁶-alkyguanine-DNA alkyltransferase assay
  • The E. coli vsr endonuclease: assaying activity in vitro and in vivo
  • Coupling of DNA helicase function to DNA strand exchange activity
  • Detection and quanitation of recBCD enzymer (exonuclease V) activity
  • Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima
  • MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance
  • pt. 2. Use of DNA repair enzymes as tools in molecular biology
  • Flexible genetic engineering using RecA protein
  • Interspecies recombination and mismatch repair: generation of mosaic genes and genomes
  • The use of immobilized mismatch binding protein in mutation/SNP detection
  • Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check)
  • Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan)
  • The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection.

Similar Items