E. coli gene expression protocols /
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Imprint: | Totawa, N.J. : Humana Press, ©2003. |
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Description: | 1 online resource (xi, 347 pages) : illustrations |
Language: | English |
Series: | Methods in molecular biology ; v. 205 Methods in molecular biology (Clifton, N.J.) ; v. 205. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11119160 |
Other authors / contributors: | Vaillancourt, Peter E. |
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ISBN: | 9781592593019 1592593011 1588290085 9781588290083 1280841885 9781280841880 9786610841882 6610841888 |
Notes: | Includes bibliographical references and index. English. |
Summary: | Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics. |
Other form: | Print version: E. coli gene expression protocols. Totawa, N.J. : Humana Press, ©2003 1588290085 |
Standard no.: | 10.1385/1592593011 |
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