Functional genomics : methods and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2003.
Description:	1 online resource (xii, 258 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology ; v. 224 Methods in molecular biology (Clifton, N.J.) ; v. 224.
Subject:	DNA microarrays -- Laboratory manuals. Genomics -- Laboratory manuals. Proteomics -- Laboratory manuals. Genomics -- methods Oligonucleotide Array Sequence Analysis -- methods DNA microarrays Genomics Proteomics Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11119957

Hidden Bibliographic Details
Other authors / contributors:	Brownstein, Michael J. Khodursky, Arkady B.
ISBN:	9781592593644 9781588292919 1588292916 159259364X 9781617374746 1588292916
Notes:	Includes bibliographical references and index.
Summary:	As high-throughput methods become more available and routine, experimentalists and theoreticians must be prepared to take advantage of the analytical power these methods offer. In Functional Genomics: Methods and Protocols, leading experimentalists-many pioneers in the field-describe in step-by-step detail robust methods for microarray-based studies and provide expert guidance in optimal data analysis and informatics. Drawing on insights derived from their long experience in biology, neurobiology, cell biology, molecular biology, and statistics, the authors review each of the key methods and analytical techniques used in functional genomics. On the methods side, this includes proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering. Additional chapters outline the many available approaches to data visualization and storage so crucial to the successful mining and examination of experimental results. Timely and cutting-edge, Functional Genomics: Methods and Protocols offers novice and advanced investigators in all biological fields a collection of highly practical, readily reproducible methods, data analysis techniques, and experimental design criteria that will ensure safe passage through one of the most demanding and fastest growing fields today.
Other form:	Print version: Functional genomics. Totowa, N.J. : Humana Press, ©2003 1588292916

Table of Contents:

Preface
Contributors
1. Fabrication of cDNA Microarrays
2. Nylon cDNA Expression Arrays
3. Plastic Microarrays: A Novel Array Support Combining the Benefits of Macro- and Microarrays
4. Preparing Fluorescent Probes for Microarray Studies
5. Escherichia coli Spotted Double-Strand DNA Microarrays: RNA Extraction, Labeling, Hybridization, Quality Control, and Data Management
6. Isolation of Polysomal RNA for Microarray Analysis
7. Parallel Analysis of Gene Copy Number and Expression Using cDNA Microarrays
8. Genome-wide Mapping of Protein-DNA Interactions by Chromatin Immunoprecipitation and DNA Microarray Hybridization
9. Statistical Issues in cDNA Microarray Data Analysis
10. Experimental Design to Make the Most of Microarray Studies
11. Statistical Methods for Identifying Differentially Expressed Genes in DNA Microarrays
12. Detecting Stable Clusters Using Principal Component Analysis
13. Clustering in Life Sciences
14. A Primer on the Visualization of Microarray Data
15. Microarray Databases: Storage and Retrieval of Microarray Data
Index

Functional genomics : methods and protocols /

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