Mitochondrial bioenergetics : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, ©2012.
Description:	1 online resource (xi, 333 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 810 Methods in molecular biology (Clifton, N.J.) ; v. 810.
Subject:	Mitochondrial pathology -- Laboratory manuals. Bioenergetics -- Laboratory manuals. Energy metabolism. Mitochondria -- metabolism Energy Metabolism Energy metabolism Bioenergetics Mitochondrial pathology Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11120238

Hidden Bibliographic Details
Other authors / contributors:	Palmeira, Carlos M. Moreno, António J. M.
ISBN:	9781617793820 1617793825 1617793817 9781617793813 9781617793813
Digital file characteristics:	text file
Notes:	Includes bibliographical references and index. English. Online resource; title from PDF title page (SpringerProtocols website, viewed Nov. 16, 2011).
Summary:	Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age. These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics.
Other form:	Printed edition: 9781617793813
Standard no.:	10.1007/978-1-61779-382-0

Table of Contents:

Overview of mitochondrial bioenergetics
Evaluation of respiration with Clark type electrode in isolated mitochondria and permeabilized animal cells
High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle
High-throughput analysis of mitochondrial oxygen consumption
Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices
Mitochondrial membrane potential ([delta][psi]) fluctuations associated with the metabolic states of mitochondria
Safranine as a fluorescent probe for the evaluation of mitochondrial membrane potential in isolated organelles and permeabilized cells
Fluorescence measurement of mitochondrial membrane potential changes in cultured cells
Phenomenological kinetic and control analysis of oxidative phosphorylation in isolated mitochondria
Expression of uncoupling proteins in a mammalian cell culture system (hek293) and assessment of their protein function
Measurement of proton leak and electron leak in isolated mitochondria
Relation between mitochondrial membrane potential and ROS formation
Use of a calcium-sensitive electrode for studies on mitochondrial calcium transport
Imaging mitochondrial calcium signalling with fluorescent probes and single or two photon confocal microscopy
Mitochondrial permeability transition pore and calcium handling
Imaging of mitochondrial pH using SNARF-1
Redox equivalents and mitochondrial bioenergetics
NMR methodologies for studying mitochondrial bioenergetics
Computational modeling of mitochondrial function.

Mitochondrial bioenergetics : methods and protocols /

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