Synthetic gene networks : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, ©2012.
Description:	1 online resource (xi, 393 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 813 Methods in molecular biology (Clifton, N.J.) ; v. 813.
Subject:	Synthetic biology -- Laboratory manuals. Bioengineering -- Laboratory manuals. Bioengineering. Regulatory Sequences, Nucleic Acid. Biology. Base Sequence. Engineering. Biological Science Disciplines. Natural Science Disciplines. Technology, Industry, and Agriculture Genetic Structures. Genetic Phenomena. Disciplines and Occupations Technology, Industry, Agriculture Phenomena and Processes Synthetic Biology. Gene Regulatory Networks. Bioengineering. Synthetic biology. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11121011

Hidden Bibliographic Details
Other authors / contributors:	Weber, Wilfried, 1974- Fussenegger, Martin.
ISBN:	9781617794124 1617794120 1617794112 9781617794117 9781617794117
Digital file characteristics:	text file
Notes:	Includes bibliographical references and index. English. Online resource; title from PDF title page (SpringerProtocols, viewed Nov. 30, 2011).
Summary:	The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology.
Other form:	Printed edition: 9781617794117
Standard no.:	10.1007/978-1-61779-412-4

Table of Contents:

In silico implementation of synthetic gene networks
Standardization in synthetic biology
Robust optimal design of synthetic biological networks
Predicting synthetic gene networks
Reprogramming a GFP reporter gene subjects it to complex lentiviral gene regulation
A high-throughput microfluidic method for generating and characterizing transcription factor mutant libraries
Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection
Zinc-finger nucleases-based genome engineering to generate isogenic human cell lines
RNA-based networks: Using RNA aptamers and ribozymes as synthetic genetic devices
MicroRNA circuits for transcriptional logic
Light-regulated gene expression in yeast
Light-controlled gene switches in mammalian cells
Expressed protein modifications: Making synthetic proteins
Using transcription machinery engineering to elicit complex cellular phenotypes
Streamlining of a pseudomonas putida genome using a combinatorial deletion method based on minitransposon insertion and the flp-FRT recombination system
Transposon-based and plasmid-based genetic tools for editing genomes of gram-negative bacteria
Synthetic networks: Oscillators and toggle switches for escherichia coli
Studying microbial communities in biofilms
Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem
Drosophila s2 schneider cells: A useful tool for rebuilding and redesigning approaches in synthetic biology
Synthetic gene networks in plant systems
Design and construction of synthetic gene networks in mammalian cells
Synthetic gene networks as blueprint for smart hydrogels.

Synthetic gene networks : methods and protocols /

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