Myogenesis : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, ©2012.
Description:	1 online resource (xv, 558 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 798 Methods in molecular biology (Clifton, N.J.) ; v. 798.
Subject:	Myogenesis -- Laboratory manuals. Publication Formats Muscles. Muscle, Striated. Musculoskeletal Development. Musculoskeletal System. Musculoskeletal Physiological Phenomena. Publication Characteristics Organogenesis. Anatomy. Tissues. Embryonic and Fetal Development Morphogenesis. Musculoskeletal and Neural Physiological Phenomena Phenomena and Processes Growth and Development. Physiological Phenomena. Muscle, Skeletal. Muscle Development. Laboratory Manuals Myogenesis. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11121454

Hidden Bibliographic Details
Other authors / contributors:	DiMario, Joseph X.
ISBN:	9781617793431 1617793434 1617793426 9781617793424 9781617793424
Digital file characteristics:	text file
Notes:	Includes index. English. Print version record.
Summary:	Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology. Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells. Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume. Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.
Other form:	Print version: Myogenesis. New York Humana Press ; Springer, ©2012 9781617793424
Standard no.:	10.1007/978-1-61779-343-1

Table of Contents:

Isolation and characterization of human fetal myoblasts
Skeletal muscle satellite cells: Background and methods for isolation and analysis in a primary culture system
Isolation of muscle stem cells by fluorescence activated cell sorting cytometry
Mouse and human mesoangioblasts: Isolation and characterization from adult skeletal muscles
Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control
Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies
Somite unit chronometry to analyze teratogen phase specificity in the paraxial mesoderm
Analysis of skeletal muscle development in drosophila
Immunocytochemistry to study myogenesis in zebrafish
Immunofluorescent localization of proteins in caenorhabditis elegans muscle
Resistance loading and signaling assays for oxidative stress in rodent skeletal muscle
Analysis of skeletal muscle hypertrophy in models of increased loading
Protein overexpression in skeletal muscle using plasmid-based gene transfer to elucidate mechanisms controlling fiber size
In vivo measurement of muscle protein synthesis rate using the flooding dose technique
Recombinant adeno-associated viral vector production and purification
Generation of lentiviral vectors for use in skeletal muscle research
Generating tamoxifen-inducible cre alleles to investigate myogenesis in mice
Gene profiling studies in skeletal muscle by quantitative real-time polymerase chain reaction assay
Analysis of lipid profiles in skeletal muscles
Proteomic analysis of dystrophic muscle
Detection of calcium release via ryanodine receptors
Measurement of calcium release due to inositol trisphosphate receptors in skeletal muscle
Detection of calcium sparks in intact and permeabilized skeletal muscle fibers
Analysis of calcium transients in cardiac myocytes and assessment of the sarcoplasmic reticulum ca(2+)-ATPase contribution
Analysis of muscle gene transcription in cultured skeletal muscle cells
Analysis of fiber-type differences in reporter gene expression of [beta]-gal transgenic muscle
Determination of gene promoter activity in skeletal muscles in vivo
Determination of miRNA targets in skeletal muscle cells
ShRNA-mediated gene knockdown in skeletal muscle
Detection of NF-[kappa]B activity in skeletal muscle cells by electrophoretic mobility shift analysis
Isolation of nuclei from skeletal muscle satellite cells and myofibers for use in chromatin immunoprecipitation assays
An improved restriction enzyme accessibility assay for analyzing changes in chromatin structure in samples of limited cell number
ChIP-enriched in silico targets (ChEST), a ChIP-on-ChIP approach applied to analyzing skeletal muscle genes.

Myogenesis : methods and protocols /

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