Arachidonate related lipid mediators /

Arachidonate related lipid mediators /

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Bibliographic Details
Imprint:San Diego : Academic Press, ©1990.
Description:1 online resource (xxxviii, 683 pages) : illustrations.
Language:English
Series:Methods in enzymology ; v. 187
Methods in enzymology ; v. 187.
Subject:
Eicosanoic acid.
Prostaglandins.
Leukotrienes.
Platelet activating factor.
Arachidonic Acids.
Lipid Metabolism.
Eicosanoic acid.
Leukotrienes.
Platelet activating factor.
Prostaglandins.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11125569
Hidden Bibliographic Details
Other authors / contributors:Murphy, Robert C.
Fitzpatrick, Frank A.
ISBN:0121820882
9780121820886
Notes:Includes bibliographical references and indexes.
Restrictions unspecified
Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2011.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
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Print version record.
Summary:This volume includes information on arachidonic acid metabolites and platelet activating factor. Assays for the quantitative measurement of prostaglandins, leukotrienes, and platelet-activating factor, including enzyme immunoassays and mass spectrophotometric techniques, are presented. Preparation and analysis of cytochrome P-450 metabolites of arachidonic acid are discussed. The isolation, purification, and assay of enzymes important in the biosynthesis and metabolism of these lipids are included.
Other form:Print version:
Table of Contents:
  • Nomenclature
  • Assays: Prostaglandins
  • Electron-Capture Negative-Ion Chemical Ionization Mass Spectrometry of Lipid Mediators
  • Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase
  • Radioimmunoassay of 11-Dehydrothromboxane B2
  • Measurement of Thromboxane Metabolites by Gas Chromatography-Mass Spectrometry
  • Quantification of Prostaglandin F2 Stable Isotope Dilution Mass Spectrometric Assay
  • Immunoaffinity Purification-Chromatographic Quantitative Analysis of Arachidonic Acid Metabolites
  • Leukotrienes
  • Preparation of Tetradeuterated Leukotriene A4 Methyl Ester: Methyl-[11,12,14,15-2H4]-(5S,6S)-Oxide-(7E,9E,11Z,14Z)-Eicosatetraenoate
  • Quantitative Gas Chromatography-Mass Spectrometry Analysis of Leukotriene B4
  • Enzyme Immunoassays for Leukotrienes C4 and E4 Using Acetylcholinesterase
  • Quantitation of Sulfidopeptide Leukotrienes in Biological Fluids by Gas Chromatography-Mass Spectrometry
  • Automated On-Line Extraction and Profiling of Lipoxygenase Products of Arachidonic Acid by High-Performance Liquid Chromatography
  • Preparation of Antibodies Directed against Leukotrienes
  • Platelet-Activating Factor
  • Bioassay of Platelet-Activating Factor Acether by Rabbit Platelet Aggregation
  • Bioassay of Platelet-Activating Factor by Release of [3H]Serotonin
  • Quantitation of Platelet-Activating Factor by Gas Chromatography-Mass Spectrometry
  • Quantitative Analysis of Platelet-Activating Factor by Gas Chromatography-Negative-Ion Chemical Ionization Mass Spectrometry
  • Isolation of Platelet-Activating Factor and Purification by Thin-Layer Chromatography
  • Separation and Characterization of Arachidonate-Containing Phosphoglycerides
  • Other Oxidative Products
  • High-Performance Liquid Chromatography Separation and Determination of Lipoxins
  • Quantitation of Epoxy- and Dihydroxyeicosatrienoic Acids by Stable Isotope-Dilution Mass Spectrometry
  • High-Performance Liquid Chromatography for Chiral Analysis of Eicosanoids
  • Phospholipase A2 and Phospholipids
  • Extraction of Phospholipids and Analysis of Phospholipid Molecular Species
  • Macrophage Phospholipase A2 Specific for sn-2-Arachidonic Acid
  • Measurement of Phosphoinositide-Specific Phospholipase C Activity
  • Solubilization of Arachidonate CoA Ligase from Cell Membranes, Chromatographic Separation from Nonspecific Long-Chain-Fatty-Acid-CoA

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