Chromosome structural analysis : a practical approach /

Chromosome structural analysis : a practical approach /

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Bibliographic Details
Imprint:	Oxford ; New York : Oxford University Press, 1999.
Description:	1 online resource (xxiii, 209 pages) : illustrations (some color)
Language:	English
Series:	The practical approach series ; 200 Practical approach series ; 200.
Subject:	Chromosomes -- Laboratory manuals. Genetics -- Laboratory manuals. Chromosomes -- genetics. Chromosomes -- ultrastructure. Chromosomes. Genetics. Laboratory Manual. Laboratory manuals. Electronic books.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11127774

Hidden Bibliographic Details
Other authors / contributors:	Bickmore, Wendy.
ISBN:	0585484090 9780585484099 1280375590 9781280375590 9786610375592 6610375593 9786610756391 6610756392 0199636990 9780199636990 0199636982 9780199636983 9780191565809 0191565806
Notes:	Includes bibliographical references and index. Restrictions unspecified Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 digitized 2010 HathiTrust Digital Library committed to preserve Print version record.
Summary:	The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (for example, histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein: DNA interactions "in vivo" using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei "in vitro."
Other form:	Print version: Chromosome structural analysis. Oxford ; New York : Oxford University Press, 1999 0199636990 0199636982

Description
Summary:	In eukaryotes, DNA is packaged in chromosomes, the structure and number of which provide a great deal of information for many aspects of cellular genetics. This hands-on guide is an up-to-date survey of experimental methods for analyzing chromosome structure, and it includes techniques for vertebrates, Drosophila, yeast, and Xenopus. Organized in a methodical fashion, the book begins with the analysis of chromatin and protein mapping, followed by chapters on whole chromosome structure, then on whole-mount tissues and the analysis of sub-structure in mammals, and concluding with two chapters on the experimental manipulation of chromosome structure. With over 70 protocols, the book will be invaluable for a wide range of researchers in genetics, medicine, and molecular biology.
Physical Description:	1 online resource (xxiii, 209 pages) : illustrations (some color)
Format:	Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Bibliography:	Includes bibliographical references and index.
ISBN:	0585484090 9780585484099 1280375590 9781280375590 9786610375592 6610375593 9786610756391 6610756392 0199636990 9780199636990 0199636982 9780199636983 9780191565809 0191565806