PCR 3 : PCR in situ hybridization : practical approach /

PCR 3 : PCR in situ hybridization : practical approach /

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Bibliographic Details
Imprint:	Oxford ; New York : IRL Press at Oxford University Press, ©1998.
Description:	1 online resource (xviii, 205 pages) : illustrations (some color).
Language:	English
Series:	Practical approach series ; 186 Practical approach series ; 186.
Subject:	In situ hybridization -- Laboratory manuals. Polymerase chain reaction -- Laboratory manuals. In situ hybridization. Polymerase chain reaction. Situ Hybridization -- Laboratory Manuals. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11127814

Hidden Bibliographic Details
Varying Form of Title:	PCR in situ hybridization PCR three
Other authors / contributors:	Herrington, C. S. O'Leary, John J.
ISBN:	0585484163 9780585484167 1280375418 9781280375415
Notes:	Includes bibliographical references and index. Print version record.
Summary:	PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads.; There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
Other form:	Print version: PCR 3. Oxford ; New York : IRL Press at Oxford University Press, ©1998 0199636338 019963632X

Description
Summary:	PCR 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
Physical Description:	1 online resource (xviii, 205 pages) : illustrations (some color).
Bibliography:	Includes bibliographical references and index.
ISBN:	0585484163 9780585484167 1280375418 9781280375415