Growth factors and receptors : a practical approach /

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Bibliographic Details
Imprint:	Oxford [England] ; New York : Oxford University Press, 1998.
Description:	1 online resource (xxii, 266 pages) : illustrations.
Language:	English
Series:	Practical approach series ; 194 Practical approach series ; 194.
Subject:	Growth factors -- Laboratory manuals. Growth factors -- Receptors -- Laboratory manuals. Genetic engineering -- Laboratory manuals. Gene therapy -- Research -- Laboratory manuals. Gene therapy -- Research. Genetic engineering. Growth factors. Growth factors -- Receptors. Growth Substances -- Laboratory Manuals. Genetic Therapy -- Laboratory Manuals. Receptors, Growth Factor -- Laboratory Manuals. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11127865

Hidden Bibliographic Details
Other authors / contributors:	McKay, Ian (Ian A.) Brown, Kenneth D.
ISBN:	0585483973 9780585483979 9780191565656 0191565652
Notes:	Includes bibliographical references and index. Print version record.
Summary:	Growth factors are extra-cellular molecules that interact with receptors on the cell surface to initiate a sequence of events culminating in transcription of DNA prior to cell division. Disturbances in the production of growth factors or the sensitivity of their receptors are important in tumour growth. Therefore, there is much interest in engineering both growth factors and their receptors to intervene in cancer. This book describes a variety of techniques for theengineering, analysis, definition of function and detection of gene expression of growth factors and their receptors. A number of p.
Other form:	Print version: Growth factors and receptors. Oxford [England] ; New York : Oxford University Press, 1998 0199636478 019963646X

Table of Contents:

Intro; Contents; List of contributors; Abbreviations; 1. Engineered growth factors and receptors, and their applications; 1. Introduction; Known growth factors and receptors; Modes of factor-receptor interaction; 2. Applications of engineered growth factors and receptors; 3. Principal methods for engineering growth factors and their receptors; Mutagenesis; Formation of chimeric proteins; Manufacture of alternative ligands and receptors; 4. Design of engineered growth factors and receptors; DNA sequence of factor or receptor to be engineered.
Analysis of existing DNA sequence for useful restriction sitesDesign of wholly synthetic growth factors and receptors; 5. Expression of DNAs encoding growth factors or receptors; Deciding on an expression system; Microbial expression; In vitro expression; Eukaryotic gene expression; Acknowledgements; References; 2. Engineering of novel neurotrophins; 1. Introduction; 2. Structure-function analyses of neurotrophins; 3. Site-directed mutagenesis; Kunkel method; PCR-based mutagenesis; 4. Expression of recombinant protein; COS cell expression; Baculovirus expression.

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