Monoclonal antibodies : a practical approach /

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Bibliographic Details
Imprint:	Oxford ; New York : Oxford University Press, 2000.
Description:	1 online resource (xxix, 479 pages) : illustrations.
Language:	English
Series:	Practical approach series Practical approach series.
Subject:	Monoclonal antibodies -- Laboratory manuals. Antibodies, Monoclonal. Immunologic Techniques. Monoclonal antibodies. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11128025

Hidden Bibliographic Details
Other authors / contributors:	Shepherd, Philip. Dean, Christopher (Christopher J.)
ISBN:	0585483566 9780585483566
Notes:	Includes bibliographical references and index. Print version record.
Summary:	Monoclonal Antibodies: A Practical Approach deals with the preparation, testing, derivation, and applications of monoclonal antibodies. It will be an invaluable laboratory bench companion to both new and experienced immunologists.
Other form:	Print version: Monoclonal antibodies. Oxford ; New York : Oxford University Press, 2000 0199637237 0199637229

Table of Contents:

List of protocols
Abbreviations
1. Preparation of Rodent Monoclonal Antibodies by in vitro Somatic Hybridization
2. Preparation of Recombinant Antibodies From Immune Rodent Spleens and the Design of Their Humanisation by CDR Grafting
Appendix
3. Selection of Antibodies From Phage Libraries of Immunoglobulin Genes
4. Arm Complexes for in vitro Display and Evolution of Antibody Combining Sites
5. Human Monoclonal Antibodies to Blood Group Antigens
6. Laboratory Based Methods for Small-Scale Productions of Monoclonal Antibodies
7. Isolation and Purification of Antibodies From Tissue Culture Supernatant, Geoff Hale
Antibody Production in Plants
8.
9. Radiolabelling of Monoclonal Antibodies
10. Non-Radioactive Antibody Probes
11. Immunogold Probes for Light and Electron Microscopy
12. Characterization of Cellular Antigens Using Monoclonal Antibodies
13. Immunoassays
14. Immunoaffinity Chromatography of Macromolecules
15. Immunochemical Detection of BrdUrd-Labelled Nuclei for Monitoring Cell Kinetics
16. Immunofluorescence Microscopy
17. FACS Analysis of Clinical Haematological Samples in Transplantation for Cancer
18. Immunocytochemical Staining of Cells and Tissues for Diagnostic Applications
19. Detection of Chemically Modified DNA in Lymphocytes of Patients Undergoing Chemotheraphy
20. Monoclonal Antibody Therapy in Organ Transplantation
21. Monoclonal Antibody Therapy in Rheumatoid Arthritis
List of suppliers
Index

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