DNA vaccines : methods and protocols /

DNA vaccines : methods and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2000.
Description:	1 online resource (xix, 529 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine ; 29 Methods in molecular medicine ; 29.
Subject:	DNA vaccines -- Research -- Methodology. Vaccines, DNA. Research Design. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11128145

Hidden Bibliographic Details
Other authors / contributors:	Lowrie, Douglas B. Whalen, Robert G.
ISBN:	9781592596881 1592596886 0896035808 9780896035805 1280831626 9781280831621 9786610831623 6610831629
Notes:	Source of title; title from title screen. Includes bibliographical references and index. English. Print version record.
Summary:	In DNA Vaccines: Methods and Protocols, state-of-the-art review articles by leading experts summarize how to develop and employ the highly promising new DNA vaccines, what clinical results can be expected from their use, and what is known about how they work. Key topics range from vaccine design and construction to preparation and delivery methods, including the use of classical adjuvants, "genetic adjuvants," and the immunostimulatory properties of DNA and selected oligonucleotide sequences. Several contributors provide strategic ideas on antigen engineering and describe the particularly novel applications of DNA vaccine methodology that have recently emerged. In addition, there is a discussion of dendritic cells and antigen-presenting cells, the understanding of whose actions holds great promise for modulating the immune response, and thus for treating disease. Cutting-edge and comprehensive, DNA Vaccines: Methods and Protocols provides a broad panorama of the methods and thinking from which the vaccines of tomorrow will evolve, and so constitutes an invaluable sourcebook for both experts developing new applications and newcomers who want to gain mastery of the techniques and problems involved.
Other form:	DNA vaccine
Standard no.:	10.1385/1592596886

Description
Summary:	The field of DNA vaccines has undergone explosive growth in the last few years. As usual, some historical precursors of this approach can be d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that genes carried by pure plasmid DNA and injected in a saline solution, hence the epithet "naked DNA," could be taken up and expressed by skeletal muscle cells with a low but reproducible frequency. Such a simple methodology was sure to spawn many applications. In a separate and important line of experimentation, Tang, De Vit, and Johnston announced in 1992 that it was indeed possible to obtain humoral immune responses against proteins encoded by DNA delivered to the skin by a biolistic device, which has colloquially become known as the "gene gun. " The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune responses to viral antigens expressed from injected plasmid DNA. In some cases, protection against challenge with the pathogen was obtained. The latter result was - questionably the touchstone of a method of vaccination worthy of the name.
Item Description:	Source of title; title from title screen.
Physical Description:	1 online resource (xix, 529 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	9781592596881 1592596886 0896035808 9780896035805 1280831626 9781280831621 9786610831623 6610831629