DNA vaccines : methods and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2000.
Description:	1 online resource (xix, 529 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine ; 29 Methods in molecular medicine ; 29.
Subject:	DNA vaccines -- Research -- Methodology. Vaccines, DNA. Research Design. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11128145

Hidden Bibliographic Details
Other authors / contributors:	Lowrie, Douglas B. Whalen, Robert G.
ISBN:	9781592596881 1592596886 0896035808 9780896035805 1280831626 9781280831621 9786610831623 6610831629
Notes:	Source of title; title from title screen. Includes bibliographical references and index. English. Print version record.
Summary:	In DNA Vaccines: Methods and Protocols, state-of-the-art review articles by leading experts summarize how to develop and employ the highly promising new DNA vaccines, what clinical results can be expected from their use, and what is known about how they work. Key topics range from vaccine design and construction to preparation and delivery methods, including the use of classical adjuvants, "genetic adjuvants," and the immunostimulatory properties of DNA and selected oligonucleotide sequences. Several contributors provide strategic ideas on antigen engineering and describe the particularly novel applications of DNA vaccine methodology that have recently emerged. In addition, there is a discussion of dendritic cells and antigen-presenting cells, the understanding of whose actions holds great promise for modulating the immune response, and thus for treating disease. Cutting-edge and comprehensive, DNA Vaccines: Methods and Protocols provides a broad panorama of the methods and thinking from which the vaccines of tomorrow will evolve, and so constitutes an invaluable sourcebook for both experts developing new applications and newcomers who want to gain mastery of the techniques and problems involved.
Other form:	DNA vaccine
Standard no.:	10.1385/1592596886

Table of Contents:

DNA vaccine design / Janet L. Brandsma
Design of plasmid DNA constructs for vaccines / Donna L. Montgomery and Kristala Jones Prather
Vaccination with messenger RNA / Stave Pascolo
A stress protein-facilitated antigen expression system for plasmid DNA vaccines / Petra Riedl [and others]
In vitro assay of immunostimulatory activities of plasmid vectors / Weiwn Jiang, Charles F. Reich, and David S. Pisetsky
Delivery of DNA vaccines using electroporation / Shawn Babiuk, Sylvia van Drunen Littel-van den Hurk, and Lorne A. Babiuk
Needle-free injection of DNA vaccines: a brief overview and methodology / Kanakatte Raviprakash and Kevin R. Porter
Needle-free delivery of veterinary DNA vaccines / Sylvia van Drunen Littel-van den Hurk, Shawn Babiuk, and Lorne A. Babiuk
Surface-modified biodegradable microspheres for DNA vaccine delivery / Mark E. Keegan and W. Mark Saltzman
A dendrimer-like DNA-based vector for DNA delivery: a viral and nonviral hybrid approach / Dan Luo [and others]
Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence / Anne Doody and David Putnam
Adjuvant properties of CpG oligonucleotides in primates / Daniela Verthelyi
Complexes of DNA vaccines with cationic, antigenic peptides are potent, polyvalent CD8+ T-cell stimulating immunogens / Petra Riedl, Jörg Reimann, and Reinhold Schirmbeck
Prime-boost strategies in DNA vaccines / C. Jane Dale [and others]
Modifying professional antigen-presenting cells to enhance DNA vaccine potency / Chien-Fu Hung, Mu Yang, and T.C. Wu
Replicase-based DNA vaccines for allergy treatment / Sandra Scheiblhofer [and others]
Immunological responses of neonates and infants to DNA vaccines / Martha Sedegah and Stepen L. Hoffman
DNA vaccines for allergy treatment / Richard Weiss, Sandra Sheiblhofer, and Josef Thalhamer
Protection from autoimmunity by DNA vaccination against T-cell receptor / Thorsten Buch and Ari Waisman
The use of bone marrow-chimeric mice in elucidating immune mechanisms / Akiko Iwasaki
A simple method for the production of plasmid DNA in bioreactors / Kristin Listner, Laura Kizer Bentley, and Michel Chartrain
Practical methods for supercoiled pDNA production / John Ballantyne
Production of plasmid DNA in industrial quantities according to cGMP guidelines / Joachim Schorr [and others]
Large-scale, nonchromatographic purification of plasmid DNA / Jason C. Murphy, Michael A. Winters, and Sangeetha L. Sagar
Assuring the quality, safety, and efficacy of DNA vaccines / James S. Robertson and Elwyn Griffiths.

DNA vaccines : methods and protocols /

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