Cancer cell culture : methods and protocols /

Cancer cell culture : methods and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (xii, 360 pages) : illustrations
Language:English
Series:Methods in molecular medicine, 1543-1894 ; 88
Methods in molecular medicine ; 88.
Subject:
Cancer cells -- Laboratory manuals.
Tumor Cells, Cultured.
Laboratory Techniques and Procedures
Cancer cells.
Laboratory Manual.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128338
Hidden Bibliographic Details
Other authors / contributors:Langdon, Simon P.
ISBN:9781592594061
1588290794
9781588290793
1592594069
1280842792
9781280842795
9786610842797
6610842795
9781617373435
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:The growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. Suitable for novice and experienced researchers alike, these readily reproducible techniques solve a diverse range of experimental problems. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the coculture of different cell types; and the detection and treatment of contamination. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cancer Cell Culture: Methods and Protocols provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories.
Other form:Print version: Cancer cell culture. Totowa, N.J. : Humana Press, ©2004 1588290794
Standard no.:10.1385/1592594069.
Table of Contents:
  • Preface
  • Contributors
  • Part I. Introduction to Cancer Cell Culture
  • 1. Basic Principles of Cancer Cell Culture
  • 2. Essential Techniques of Cancer Cell Culture
  • Part II. Characterization and Authentication
  • 3. Characterization and Authentication of Cancer Cell Lines: An Overview
  • 4. Authentication of Cancer Cell Lines by DNA Fingerprinting
  • 5. Cytogenetic Characterization of Tumor Cell Lines
  • Part III. Isolation and Culture of Specialized Cell Types
  • 6. Isolation and Culture of Colon Cancer Cell Lines
  • 7. Isolation and Culture of Melanoma Cell Lines
  • 8. Isolation and Culture of Human Brain Tumor Cells
  • 9. Isolation and Culture of Renal Cancer Cell Lines
  • 10. Isolation and Culture of Prostate Cancer Cell Lines
  • 11. Isolation and Culture of Ovarian Cancer Cell Lines
  • 12. Isolation and Culture of Leukemia Cell Lines
  • Part IV. Functional Assays in Cell Culture
  • 13. Cell Sensitivity Assays: Clonogenic Assay
  • 14. Cell Sensitivity Assays: The MTT Assay
  • 15. PARP Cleavage as a Means of Assessing Apoptosis
  • 16. Detection of Apoptosis by the TUNEL Assay
  • 17. Apoptosis Measurement by Annexin V Staining
  • 18. Cell Adhesion Assays
  • 19. Radial Monolayer Cell Migration Assay
  • 20. Invasion and Motility Assays
  • 21. Identification of Senescence in Cancer Cells
  • 22. Angiogenesis Assays
  • 23. Flow Cytometric DNA Analysis of Human Cancer Cell Lines
  • Part V. Modification of Cancer Cells
  • 24. DNA-Mediated Gene Transfer
  • 25. Development of Drug-Resistant Models
  • 26. Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
  • 27. The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts
  • Part VI. Coculture Systems
  • 28. Coculture of Prostate Cancer Cells
  • 29. Coculture of Ovarian Cells Using Porous Tissue Culture Inserts
  • Part VII. Cell Line Contamination
  • 30. Cell Culture Contamination: An Overview
  • 31. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
  • 32. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
  • Appendix 1. Formulations of Commonly Used Cell Culture Media
  • Appendix 2. Human Cancer Cell Lines Available from the ATCC and DSMZ Cell Banks (Combined List)
  • Index

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