Vaccine protocols /

Vaccine protocols /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, N.J. : Humana Press, ©2003.
Description:	1 online resource (xi, 414 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine ; 87 Methods in molecular medicine ; 87.
Subject:	Vaccines -- Laboratory manuals. Vaccines. Drug Design. Genetic Techniques. Research Design. Vaccines. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11128347

Hidden Bibliographic Details
Other authors / contributors:	Robinson, Andrew, 1947- Hudson, Michael J. Cranage, Martin P.
ISBN:	9781592593996 1588291405 9781588291400 1592593992 1280842733 9781280842733 9786610842735 6610842736 9781617373824 1617373826 9781617373824 9781489938251 1489938257
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.
Other form:	Print version: Vaccine protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2003 1588291405
Standard no.:	10.1385/1592593992.

Description
Summary:	Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms. The aim of this volume is to provide a selection of contemporary protocols that will be useful to both novice and advanced practitioner alike. The variety of procedures required to design, develop, produce, and assess a vaccine is immense and covers aspects of ch- istry, biochemistry, molecular biology, cell biology, and immunology. No single volume can hope to cover these topics exclusively. Rather, here we attempt to provide a methods sourcebook focusing on hands-on practical advice. Comp- mentary and background information may be found in other volumes in the Me- ods in Molecular Medicine series. Of particular interest are volumes on Dendritic Cell Protocols, Interleukin Protocols, Vaccine Adjuvants, and DNA Vaccines. Since the publication of the first edition of Vaccine Protocols there have been major advances, particularly in the areas of bacterial genomics, antig- specific T-cell quantification, genetic manipulation of vaccine vectors, the h- nessing of natural molecules concerned with the regulation of immune responses, and the burgeoning field of DNA vaccinology. Hence, the extensive revision of this edition with new chapters on live viral vaccine vectors, atte- ated bacterial vectors, immunomodulators, MHC-peptide tetrameric complexes, and the identification of vaccine candidates by genomic analysis. Additionally, chapters from the first edition have been updated to accommodate state-of-t- art methods in vaccinology.
Physical Description:	1 online resource (xi, 414 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	9781592593996 1588291405 9781588291400 1592593992 1280842733 9781280842733 9786610842735 6610842736 9781617373824 1617373826 9781489938251 1489938257