Vaccine protocols /

Vaccine protocols /

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Bibliographic Details
Edition:2nd ed.
Imprint:Totowa, N.J. : Humana Press, ©2003.
Description:1 online resource (xi, 414 pages) : illustrations
Language:English
Series:Methods in molecular medicine ; 87
Methods in molecular medicine ; 87.
Subject:
Vaccines -- Laboratory manuals.
Vaccines.
Drug Design.
Genetic Techniques.
Research Design.
Vaccines.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128347
Hidden Bibliographic Details
Other authors / contributors:Robinson, Andrew, 1947-
Hudson, Michael J.
Cranage, Martin P.
ISBN:9781592593996
1588291405
9781588291400
1592593992
1280842733
9781280842733
9786610842735
6610842736
9781617373824
1617373826
9781617373824
9781489938251
1489938257
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.
Other form:Print version: Vaccine protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2003 1588291405
Standard no.:10.1385/1592593992.
Table of Contents:
  • Overview of vaccines / G. Ada
  • Temperature-sensitive mutant vaccines / C.R. Pringle
  • Live viral vectors: construction of a replication-deficient recombinant adenovirus / A.R. Fooks
  • Live viral vectors: vaccinia virus / C. Staib and G. Sutter
  • Live viral vectors: Semliki Forest virus / G.B. Karlsson and P. Liljestrom
  • Development of attenuated Salmonella strains that express heterologous antigens / F. Bowe [and others]
  • Expression and delivery of heterologous antigens using lactic acid bacteria / M.A. Reuter, S. Hanniffy and J.M. Wells
  • Synthetic peptides / M.J. Francis
  • Genetic detoxification of bacterial toxins / M. Pizza [and others]
  • Preparation of polysaccharide-conjugate vaccines / C.C. Peeters [and others]
  • Adjuvant formulations for experimental vaccines / D.E. Stewart-Tull
  • Incorporation of immunomodulators into plasmid DNA vaccines / L.S. Klavinskis, P. Hobson and A. Woods
  • Microencapsulation of vaccine antigens / D.H. Jones
  • Lyophilization of vaccines: current trends / G.D. Adams
  • Stimulation of mucosal immunity / D.J. Lewis and C.M. Hayward
  • Induction and detection of T-cell responses / K.H. Mills
  • Construction of MHC class I-peptide tetrameric complexes for analysis of T-cell-mediated immune responses / R.V. Samuel and T. Hanke
  • Assessment of functional antibody responses / R. Borrow and P. Balmer
  • The use of complete genome sequences in vaccine design / N.J. Saunders and S. Butcher
  • Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment / M.J. Dennis
  • Clinical trials / P. Farrington and E. Miller
  • Assuring the quality and safety of vaccines: regulatory licensing and batch release / E. Griffiths and I. Knezevic
  • DNA vaccination: an update / D.B. Lowrie
  • From vaccine research to manufacture: a guide for the researcher / N. Allison and H.S. Tranter.

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