Mycobacterium tuberculosis protocols /

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Bibliographic Details
Imprint:Totowa, NJ : Humana Press, ©2001.
Description:1 online resource (xiv, 403 pages) : illustrations
Language:English
Series:Methods in molecular medicine ; 54
Methods in molecular medicine ; 54.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128699
Hidden Bibliographic Details
Other authors / contributors:Parish, Tanya.
Stoker, Neil G.
ISBN:9781592591473
0896037762
9780896037762
1592591477
1280821124
9781280821127
9786610821129
6610821127
9781617371844
Notes:Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become acute. In Mycobacterium tuberculosis Protocols, leading investigators with extensive practical knowledge and experience describe their best methods for studying this dangerous pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. These molecular techniques are suitable for research in genetics, biochemistry, microbiology, cell biology, epidemiology, and diagnostics, and are at the forefront of biological research as a whole, as well as in focused M. tuberculosis research. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start-site mapping, real-time PCR, microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.
Other form:Mycobacterium tuberculosis protocols
Online version: Mycobacterium tuberculosis protocols. Totowa, NJ : Humana Press, ©2001
Standard no.:10.1385/1592591477