MRNA processing and metabolism : methods and protocols /

Saved in:
Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (xv, 270 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 257
Methods in molecular biology (Clifton, N.J.) ; v. 257.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128705
Hidden Bibliographic Details
Other authors / contributors:Schoenberg, Daniel R.
ISBN:9781592597505
1588292258
9781588292254
1592597505
128036047X
9781280360473
9786610360475
6610360472
9781617374340
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:MRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.
Other form:MRNA processing and metabolism
Standard no.:10.1385/1592597505.