Protocols for nucleic acid analysis by nonradioactive probes /

Protocols for nucleic acid analysis by nonradioactive probes /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©1994.
Description:	1 online resource (xii, 268 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; v. 28 Methods in molecular biology (Clifton, N.J.) ; v. 28.
Subject:	Nucleic acid probes. Nucleic acids -- Analysis. Nucleic Acid Probes Nucleic Acids -- analysis Nucleic acid probes Nucleic acids -- Analysis Laboratory Manual Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11129079

Hidden Bibliographic Details
Varying Form of Title:	Nonradioactive probes
Other authors / contributors:	Isaac, Peter G.
ISBN:	089603254X 9780896032545 9781592595150 1592595154
Notes:	Source of title; title from title screen. Includes bibliographical references and index. English.
Summary:	This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.
Other form:	Protocols for nucleic acid analysis by nonradioactive probes
Standard no.:	10.1385/089603254X.

Description
Summary:	This book offers tried and tested protocols for analyzing nucleic acids without the use of radioactive probes. It also covers Southern (DNA) blot and Northern (RNA) blotting protocols in detail, from how to isolate good quality nucleic acids, through the use of the widest range of probe systems involving digoxigenin, biotin, fluorescein, horseradish peroxidase labelling, and colorimetric and chemiluminescent detection. Also included are novel techniques such as RAPD, reverse dot blot, and nucleic acid sequence-based amplification.
Item Description:	Source of title; title from title screen.
Physical Description:	1 online resource (xii, 268 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	089603254X 9780896032545 9781592595150 1592595154