Protocols for nucleic acid analysis by nonradioactive probes /
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Imprint: | Totowa, N.J. : Humana Press, ©1994. |
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Description: | 1 online resource (xii, 268 pages) : illustrations |
Language: | English |
Series: | Methods in molecular biology ; v. 28 Methods in molecular biology (Clifton, N.J.) ; v. 28. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11129079 |
Summary: | This book offers tried and tested protocols for analyzing nucleic acids without the use of radioactive probes. It also covers Southern (DNA) blot and Northern (RNA) blotting protocols in detail, from how to isolate good quality nucleic acids, through the use of the widest range of probe systems involving digoxigenin, biotin, fluorescein, horseradish peroxidase labelling, and colorimetric and chemiluminescent detection. Also included are novel techniques such as RAPD, reverse dot blot, and nucleic acid sequence-based amplification. |
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Item Description: | Source of title; title from title screen. |
Physical Description: | 1 online resource (xii, 268 pages) : illustrations |
Bibliography: | Includes bibliographical references and index. |
ISBN: | 089603254X 9780896032545 9781592595150 1592595154 |