Signal transduction protocols /
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| Imprint: | Totowa, N.J. : Humana Press, ©1995. |
|---|---|
| Description: | 1 online resource (xi, 305 pages) : illustrations. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 41 Methods in molecular biology (Clifton, N.J.) ; v. 41. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11129127 |
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| 245 | 0 | 0 | |a Signal transduction protocols / |c edited by David A. Kendall and Steven J. Hill. |
| 260 | |a Totowa, N.J. : |b Humana Press, |c ©1995. | ||
| 300 | |a 1 online resource (xi, 305 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology ; |v v. 41 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a The problems and pitfalls of radioligand binding / Mary Keen -- Solubilization and purification of a functional ionotropic excitatory amino acid receptor / Jeremy M. Henley, David M. Kirkham -- Autoradiography of [beta]₁- and [beta]₂-adrenoceptors / Roger J. Summers, Peter Molenaar -- In situ hybridization / Brian J. Morris -- Measurement of the GTPase activity of signal-transducing G-proteins in neuronal membranes / Marva Sweeney -- Measurement of adenylyl cyclase activity in cell membranes / Robert J. Williams, Eamonn Kelly -- The measurement of cyclic AMP levels in biological preparations / Stephen P.H. Alexander -- Mass measurements of cyclic AMP formation by radioimmunoassay, enzyme immunoassay and scintillation proximity assay / Jeffrey K. Horton, Peter M. Baxendale -- Measurement of cyclic GMP formation / Felix Hernandez -- Cyclic AMP-dependent protein kinase activity ratio assay / Kenneth J. Murray -- Purification and activation of cyclic GMP-dependent protein kinase / Takayoshi Kuno, Hideyuki Mukai -- The analysis and assay of cyclic nucleotide phosphodiesterase isoenzyme activity / Mohammed Shahid, C. David Nicholson -- Separation of labeled inositol phosphate isomers by high-pressure liquid chromatography (HPLC) / Stephen Jenkinson -- Mass measurement of key phosphoinositide cycle intermediates / R.A. John Chaliss -- Measurement of phospholipase C activity in brain membranes / Enrique Claro, Elisabet Sarri, Fernando Picatoste -- Measurement of diacylglycerol : origins of receptor-stimulated diacylglycerol accumulation / Michael R. Boarder, John R. Purkiss -- Measurement of intracellular free calcium ion concentration in cell populations using fura-2 / Philip A. Iredale, John M. Dickenson -- Measurement of Ca² fluxes in permeabilized cells using ⁴⁵Ca² and fluo-3 / Robert A. Wilcox, James Strupish, Stefan R. Nahorski -- Single-cell calcium imaging / Katrina A. Marsh -- Characterization of calcium/calmodulin-stimulated protein kinase II / Paula E. Jarvie, Peter R. Dunkley -- Protein kinase C : measurement of translocation, activation and role in cellular responses / Sandra E. Wilkinson, Trevor J. Hallam -- The measurement of phospholipase D-linked signaling in cells / Michael J.O. Wakelam, Matthew Hodgkin, Ashley Martin -- Phospholipase A₂ activity / Ronald M. Burch -- Techniques for the measurement of nitric oxide / Anna M. Leone [and others]. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a This collection of self-contained, "hands-on" descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the field as well as those in related areas the opportunity to expand their range of experimental techniques and to carry out successful investigations without needing to refer to any other methodological source | ||
| 650 | 0 | |a Cellular signal transduction |x Research |x Methodology. | |
| 650 | 0 | |a G proteins |x Research |x Methodology. | |
| 650 | 0 | |a Cell receptors |x Research |x Methodology. | |
| 650 | 1 | 2 | |a GTP-Binding Proteins |x analysis |
| 650 | 1 | 2 | |a Signal Transduction |x physiology |
| 650 | 1 | 4 | |a Life Sciences. |
| 650 | 2 | 4 | |a Cell Biology. |
| 650 | 6 | |a Transduction du signal cellulaire |x Recherche |x Méthodologie. | |
| 650 | 6 | |a Protéines G |x Recherche |x Méthodologie. | |
| 650 | 6 | |a Récepteurs cellulaires |x Recherche |x Méthodologie. | |
| 650 | 7 | |a Cell receptors |x Research |x Methodology |2 fast | |
| 650 | 7 | |a Cellular signal transduction |x Research |x Methodology |2 fast | |
| 650 | 7 | |a G proteins |x Research |x Methodology |2 fast | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 4 | |a Fulltext. | |
| 655 | 4 | |a Laboratory Manuals. | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft |0 http://id.loc.gov/authorities/genreForms/gf2014026120 | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf | |
| 700 | 1 | |a Kendall, David A. |0 http://id.loc.gov/authorities/names/n95006387 | |
| 700 | 1 | |a Hill, Stephen J. |0 http://id.loc.gov/authorities/names/n95006392 | |
| 776 | 0 | 8 | |i Print version: |t Signal transduction protocols. |d Totowa, N.J. : Humana Press, ©1995 |z 0896032981 |w (DLC) 95002769 |w (OCoLC)31970440 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 41. |x 1064-3745 | |
| 856 | 4 | 0 | |u https://link.springer.com/10.1385/0896032981 |y Springer Nature |
| 880 | 0 | |6 505-00/(S |a Problems and pitfalls of radioligand binding / Mary Keen -- Solubilization and purification of a functional ionotropic excitatory amino acid receptor / Jeremy M. Henley, David M. Kirkham -- Autoradiography of β₁- and β₂-adrenoceptors / Roger J. Summers, Peter Molenaar -- In situ hybridization / Brian J. Morris -- Measurement of the GTPase activity of signal-transducing G-proteins in neuronal membranes / Marva Sweeney -- Measurement of adenylyl cyclase activity in cell membranes / Robert J. Williams, Eamonn Kelly. | |
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| 928 | |t Library of Congress classification |a QP517.C45S555 1995 |l Online |c UC-FullText |u https://link.springer.com/10.1385/0896032981 |z Springer Nature |g ebooks |i 12530695 | ||