Plant signalling networks : methods and protocols /
Saved in:
| Imprint: | New York : Humana Press, ©2012. |
|---|---|
| Description: | 1 online resource (xi, 230 pages) |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; 876 Methods in molecular biology (Clifton, N.J.) ; 876. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11132305 |
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| 245 | 0 | 0 | |a Plant signalling networks : |b methods and protocols / |c edited by Zhi-Yong Wang, Zhenbiao Yang. |
| 260 | |a New York : |b Humana Press, |c ©2012. | ||
| 300 | |a 1 online resource (xi, 230 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 876 | |
| 505 | 0 | |a Experimental Analysis of Receptor Kinase Phosphorylation -- Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis (SILIA) and Functional Phosphoproteomic Strategies -- Identification of O-GlcNAc Modified Proteins from Arabidopsis -- Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE) -- Quantitative Analysis of Plasma Proteome Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE) -- Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana -- Small-Molecule Dissection of Brassinosteroid (BR) Signaling -- A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination -- Activation Tagging -- Rho GTPase Activity Analysis in Plant Cells -- Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy -- In vivo Ubiquitination Assay by Agroinfiltration -- In Vitro Protein Ubiquitination Assay -- Genome-Wide Identification of Transcription Factor Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq) -- Smart Pooling of mRNA Samples for Efficient Transcript Profiling -- Transient Expression Assays for Quantifying Signaling Output -- Genome-Wide Profiling of Uncapped mRNA -- Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches. | |
| 520 | |a Signal transduction is the fundamental mechanism for regulation of cellular activities by environmental cues and regulatory signals, and is particularly important for plants, whose survival requires proper physiological and developmental responses to the environmental changes. Much progress has been made recently in the plant signal transduction research field thanks to the development of diverse techniques which are covered in Plant Signalling Networks: Methods and Protocols. These include advanced research methods such as proteomics and mass spectrometry methods for studying protein modification, biochemical and cell biological tools for studying protein-protein interactions, genomic techniques for dissecting protein-DNA interaction and transcription networks, and computation methods that integrate molecular network into plant developmental processes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Plant Signalling Networks: Methods and Protocols presents well-honed methodologies for a wide range of research approaches including genetics, proteomics, biochemical, cell biological, and computational approaches, and seeks to serve both professionals and novices with a comprehensive understanding of complex signaling networks in plants. | ||
| 504 | |a Includes bibliographical references and index. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Plant cellular signal transduction |v Laboratory manuals. | |
| 650 | 2 | |a Biochemical Phenomena. | |
| 650 | 2 | |a Cell Physiological Phenomena. | |
| 650 | 2 | |a Eukaryota. | |
| 650 | 2 | |a Brassicaceae. | |
| 650 | 2 | |a Proteins. | |
| 650 | 2 | |a Amino Acids, Peptides, and Proteins | |
| 650 | 2 | |a Organisms | |
| 650 | 2 | |a Magnoliopsida. | |
| 650 | 2 | |a Chemical Phenomena. | |
| 650 | 2 | |a Embryophyta. | |
| 650 | 2 | |a Chemicals and Drugs | |
| 650 | 2 | |a Phenomena and Processes | |
| 650 | 2 | |a Streptophyta. | |
| 650 | 2 | |a Viridiplantae. | |
| 650 | 2 | |a Signal Transduction. | |
| 650 | 2 | |a Arabidopsis. | |
| 650 | 2 | |a Plant Proteins. | |
| 650 | 2 | |a Plants. | |
| 650 | 7 | |a Plant cellular signal transduction. |2 fast |0 (OCoLC)fst01065327 | |
| 653 | 4 | |a Life sciences. | |
| 653 | 4 | |a Proteomics. | |
| 653 | 4 | |a Botany. | |
| 653 | 4 | |a Plant Sciences. | |
| 653 | 0 | 0 | |a planten |
| 653 | 0 | 0 | |a plants |
| 653 | 0 | 0 | |a plantencelbiologie |
| 653 | 0 | 0 | |a plant cell biology |
| 653 | 0 | 0 | |a signaaltransductie |
| 653 | 0 | 0 | |a signal transduction |
| 653 | 1 | 0 | |a Plant Cell Biology |
| 653 | 1 | 0 | |a Plantencelbiologie |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 700 | 1 | |a Wang, Zhi-Yong. | |
| 700 | 1 | |a Yang, Zhenbiao, |d 1961- |0 http://id.loc.gov/authorities/names/n2007086379 | |
| 776 | 0 | 8 | |i Print version: |t Plant signalling networks. |d New York : Humana Press, ©2012 |w (DLC) 2012936833 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v 876. | |
| 903 | |a HeVa | ||
| 929 | |a oclccm | ||
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| 928 | |t Library of Congress classification |a QK725 .P53 2012 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-61779-809-2 |z Springer Nature |g ebooks |i 12531696 | ||