Plant signalling networks : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, ©2012.
Description:	1 online resource (xi, 230 pages)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 876 Methods in molecular biology (Clifton, N.J.) ; 876.
Subject:	Plant cellular signal transduction -- Laboratory manuals. Biochemical Phenomena. Cell Physiological Phenomena. Eukaryota. Brassicaceae. Proteins. Amino Acids, Peptides, and Proteins Organisms Magnoliopsida. Chemical Phenomena. Embryophyta. Chemicals and Drugs Phenomena and Processes Streptophyta. Viridiplantae. Signal Transduction. Arabidopsis. Plant Proteins. Plants. Plant cellular signal transduction. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11132305

Hidden Bibliographic Details
Other authors / contributors:	Wang, Zhi-Yong. Yang, Zhenbiao, 1961-
ISBN:	9781617798092 1617798096 1617798088 9781617798085 9781617798085
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English.
Summary:	Signal transduction is the fundamental mechanism for regulation of cellular activities by environmental cues and regulatory signals, and is particularly important for plants, whose survival requires proper physiological and developmental responses to the environmental changes. Much progress has been made recently in the plant signal transduction research field thanks to the development of diverse techniques which are covered in Plant Signalling Networks: Methods and Protocols. These include advanced research methods such as proteomics and mass spectrometry methods for studying protein modification, biochemical and cell biological tools for studying protein-protein interactions, genomic techniques for dissecting protein-DNA interaction and transcription networks, and computation methods that integrate molecular network into plant developmental processes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Plant Signalling Networks: Methods and Protocols presents well-honed methodologies for a wide range of research approaches including genetics, proteomics, biochemical, cell biological, and computational approaches, and seeks to serve both professionals and novices with a comprehensive understanding of complex signaling networks in plants.
Other form:	Print version: Plant signalling networks. New York : Humana Press, ©2012
Standard no.:	10.1007/978-1-61779-809-2

Table of Contents:

Experimental Analysis of Receptor Kinase Phosphorylation
Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis (SILIA) and Functional Phosphoproteomic Strategies
Identification of O-GlcNAc Modified Proteins from Arabidopsis
Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
Quantitative Analysis of Plasma Proteome Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana
Small-Molecule Dissection of Brassinosteroid (BR) Signaling
A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination
Activation Tagging
Rho GTPase Activity Analysis in Plant Cells
Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy
In vivo Ubiquitination Assay by Agroinfiltration
In Vitro Protein Ubiquitination Assay
Genome-Wide Identification of Transcription Factor Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)
Smart Pooling of mRNA Samples for Efficient Transcript Profiling
Transient Expression Assays for Quantifying Signaling Output
Genome-Wide Profiling of Uncapped mRNA
Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches.

Plant signalling networks : methods and protocols /

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