Signal transduction protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, ©2004. |
| Description: | 1 online resource (xii, 327 pages) : illustrations |
| Language: | English |
| Series: | Methods in molecular biology ; 284 Methods in molecular biology (Clifton, N.J.) ; v. 284. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11135034 |
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| 245 | 0 | 0 | |a Signal transduction protocols / |c edited by Robert C. Dickson and Michael D. Mendenhall. |
| 250 | |a 2nd ed. | ||
| 260 | |a Totowa, N.J. : |b Humana Press, |c ©2004. | ||
| 300 | |a 1 online resource (xii, 327 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology ; |v 284 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- Signal transduction inhibitors in cellular function / Maofu Fu [and others] -- Two-dimensional gel electrophoresis for the identification of signaling targets -- Yukihito Kabuyama [and others] -- A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski -- Determining protein half-lives / Pengbo Zhou -- Assaying protein kinase activity / Jan Brbek and Steven K. Hanks -- Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES/MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- Measurement of protein/DNA interactions in vivo by chromatin immunoprecipitation -- Hogune Im [and others]. | |
| 505 | 0 | |a Characterization of protein-DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- Nonradioactive methods for detecting activation of ras-related small G proteins / Douglas A. Andres -- Nucleocytoplasmic glycosylation, o-glcnac: identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart -- Techniques in protein methylation / Jaeho Lee, Donghang Cheng, and Mark T. Bedford -- Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- Assaying phospholipase A2 activity / Christina C. Leslie and Michael H. Gelb -- Measurement and immunofluorescence of cellular phosphinositides / Hiroko Hama [and others] -- Measuring dynamic changes in camp using fluorescence resonance energy transfer / Sandrine Evellin [and others] -- In vivo detection of protein/protein interaction in plant cells using BRET / Chitra Subramanian [and others] -- Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- Assaying cytochrome C translocation during apoptosis / Nigel J. Waterhouse [and others]. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach. | ||
| 650 | 0 | |a Cellular signal transduction |v Laboratory manuals. | |
| 650 | 0 | |a G proteins |v Laboratory manuals. | |
| 650 | 0 | |a Cell receptors |v Laboratory manuals. | |
| 650 | 2 | |a GTP-Binding Proteins |x analysis | |
| 650 | 2 | |a Signal Transduction |x physiology | |
| 650 | 6 | |a Transduction du signal cellulaire |v Manuels de laboratoire. | |
| 650 | 6 | |a Protéines G |v Manuels de laboratoire. | |
| 650 | 6 | |a Récepteurs cellulaires |v Manuels de laboratoire. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a Cell receptors |2 fast | |
| 650 | 7 | |a Cellular signal transduction |2 fast | |
| 650 | 7 | |a G proteins |2 fast | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 4 | |a Fulltext. | |
| 655 | 4 | |a Illustration. | |
| 655 | 4 | |a Internet Resource. | |
| 655 | 4 | |a Laboratory Manuals. | |
| 655 | 7 | |a Handbooks and manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft |0 http://id.loc.gov/authorities/genreForms/gf2014026120 | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf | |
| 700 | 1 | |a Dickson, Robert C. | |
| 700 | 1 | |a Mendenhall, Michael Dean. | |
| 776 | 0 | 8 | |i Print version: |t Signal transduction protocols. |b 2nd ed. |d Totowa, N.J. : Humana Press, ©2004 |z 1588292452 |z 1592598161 |w (DLC) 2004005164 |w (OCoLC)54687127 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 284. | |
| 856 | 4 | 0 | |u https://link.springer.com/10.1385/1592598161 |y Springer Nature |
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