Capillary electrophoresis of proteins and peptides /

Capillary electrophoresis of proteins and peptides /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (xi, 332 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; v. 276 Methods in molecular biology (Clifton, N.J.) ; v. 276.
Subject:	Capillary electrophoresis -- Laboratory manuals. Proteins -- Separation -- Laboratory manuals. Peptides -- Separation -- Laboratory manuals. Electrophoresis, Capillary -- methods Proteins -- analysis Capillary electrophoresis Peptides -- Separation Proteins -- Separation Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11135037

Hidden Bibliographic Details
Other authors / contributors:	Strege, Mark A. Lagu, Avinash L.
ISBN:	1588290174 9781588290175 159259798X 9781592597987 1280358254 9781280358258 9786610358250 6610358257
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development
Other form:	Print version: Capillary electrophoresis of proteins and peptides. Totowa, N.J. : Humana Press, ©2004 1588290174 159259798X
Standard no.:	10.1385/159259798X.

Description
Summary:	Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1-4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5-7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Physical Description:	1 online resource (xi, 332 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	1588290174 9781588290175 159259798X 9781592597987 1280358254 9781280358258 9786610358250 6610358257
ISSN:	1064-3745 ;