Capillary electrophoresis of proteins and peptides /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (xi, 332 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; v. 276 Methods in molecular biology (Clifton, N.J.) ; v. 276.
Subject:	Capillary electrophoresis -- Laboratory manuals. Proteins -- Separation -- Laboratory manuals. Peptides -- Separation -- Laboratory manuals. Electrophoresis, Capillary -- methods Proteins -- analysis Capillary electrophoresis Peptides -- Separation Proteins -- Separation Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11135037

Hidden Bibliographic Details
Other authors / contributors:	Strege, Mark A. Lagu, Avinash L.
ISBN:	1588290174 9781588290175 159259798X 9781592597987 1280358254 9781280358258 9786610358250 6610358257
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development
Other form:	Print version: Capillary electrophoresis of proteins and peptides. Totowa, N.J. : Humana Press, ©2004 1588290174 159259798X
Standard no.:	10.1385/159259798X.

Table of Contents:

Preface
Contributors
1. Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis
2. Capillary Coating for Protein Separation Based on Si-O and Si-C Covalent Bond Formation for Capillary Electrophoresis With Laser-Induced Fluorescence Detection
3. On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection
4. Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications
5. Capillary Electrophoresis in the Analysis and Monitoring of Biotechnological Processes
6. Capillary Electrophoresis of Proteins in a Quality Control Environment
7. Analysis of Neutral N-Linked Oligosaccharides From Antibodies Using Free-Solution Capillary Electrophoresis in Bare Fused-Silica Capillaries
8. Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions
9. Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis
10. Using Charge Ladders and Capillary Electrophoresis to Measure the Charge, Size, and Electrostatic Interactions of Proteins
11. Frontal Analysis Continuous Capillary Electrophoresis for Protein-Polyelectrolyte Binding Studies
12. Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection
13. Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Proteins
14. Capillary Isoelectric Focusing-Mass Spectrometry of Proteins and Protein Complexes
15. Integrated System for Rapid Proteomics Analyses Using Microfluidic Devices Coupled to Nanoelectrospray Mass Spectrometry
Index

Capillary electrophoresis of proteins and peptides /

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