Receptor binding techniques /

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Bibliographic Details
Edition:	3rd ed.
Imprint:	New York, NY : Humana ; London : Springer [distributor], 2012.
Description:	1 online resource (xii, 309 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; v. 897 Methods in molecular biology (Clifton, N.J.) ; volume 897.
Subject:	Receptor-ligand complexes -- Research -- Laboratory manuals. Ligand binding (Biochemistry) -- Laboratory manuals. Cell receptors -- Research -- Laboratory manuals. Biochemical Phenomena. Proteins. Metabolism. Diagnostic Techniques, Endocrine. Diagnostic Techniques, Radioisotope. Investigative Techniques Laboratory Techniques and Procedures Receptors, Cell Surface. Molecular Structure. Membrane Proteins. Diagnostic Techniques and Procedures. Analytical, Diagnostic and Therapeutic Techniques and Equipment Amino Acids, Peptides, and Proteins Chemical Phenomena. Diagnosis. Chemicals and Drugs Phenomena and Processes Radioligand Assay. Methods. Binding Sites. Receptors, Drug. Receptors, G-Protein-Coupled. Carrier Proteins. Cell receptors -- Research. Ligand binding (Biochemistry) Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11136020

Hidden Bibliographic Details
Other authors / contributors:	Davenport, Anthony P.
ISBN:	9781617799099 1617799092 9781617799082 1617799084
Digital file characteristics:	PDF text file
Notes:	Previous edition: 2005. Includes bibliographical references and index. English. Print version record.
Summary:	A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel 'biased agonists'. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding.
Other form:	Print version: Receptor binding techniques. 3rd ed. New York, NY : Humana Press, 2012 9781617799082
Standard no.:	10.1007/978-1-61779-909-9

Table of Contents:

Receptor databases and computational websites for ligand binding / Brinda K. Rana, Philip E. Bourne, and Paul A. Insel
How to use the IUPHAR receptor database to navigate pharmacological data / Chidochangu P. Mpamhanga [and others]
Radioligand binding assays and their analysis / Janet J. Maguire, Rhoda E. Kuc, and Anthony P. Davenport
Use of scintillation proximity assay to measure radioligand binding to immobilized receptors without separation of bound from free ligand / Jenny Berry, Molly Price-Jones, and Barbara Killian
Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands / Craig J. Daly, Ingela Parmryd, and John C. McGrath
Dissecting the pharmacology of G protein-coupled receptor signaling complexes using bimolecular fluorescence complementation / Laura E. Kilpatrick and Nicholas D. Holliday
Live cell imaging of G protein-coupled receptors / Anke Teichmann [and others]
Characterization of G-protein coupled receptor modulators using homogeneous cAMP assays / Daniel L. Bassoni [and others]
Measurements of [beta]-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation / Daniel L. Bassoni [and others]
Quantitative phosphor imaging autoradiography of radioligands for positron emission tomography / Peter Johnstrom, Joseph L. Bird, and Anthony P. Davenport
Dynamic in vivo imaging of receptors in small animals using positron emission tomography / Peter Johnstrom [and others]
Cellular localization of receptors using antibodies visualized by light and dual labeling confocal microscopy / Anthony P. Davenport and Rhoda E. Kuc
Detection of mRNA encoding receptors by in situ and northern hybridization / Alessandra P. Princivalle [and others].

Receptor binding techniques /

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