Metastasis research protocols /

Saved in:

Bibliographic Details
Edition:	2nd ed.
Imprint:	New York : Humana Press, ©2012.
Description:	1 online resource (xii, 274 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; 878 Methods in molecular biology (Clifton, N.J.) ; v. 878.
Subject:	Metastasis -- Laboratory manuals. Cancer cells -- Laboratory manuals. Investigative Techniques Neoplastic Processes. Clinical Laboratory Techniques. Analytical, Diagnostic and Therapeutic Techniques and Equipment Publication Formats Pathologic Processes. Neoplasms. Publication Characteristics Pathological Conditions, Signs and Symptoms Disease. Cell Transformation, Neoplastic. Cytological Techniques. Neoplasm Metastasis. Laboratory Manuals Methods. Diagnosis. Cancer cells. Metastasis. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11136284

Hidden Bibliographic Details
Other authors / contributors:	Dwek, M. V. (Miriam V.) Brooks, Susan A. Schumacher, Udo.
ISBN:	9781617798542 1617798541 1617798533 9781617798535 9781617798535
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Online resource; title from PDF title page (Springer Protocols, viewed October 17, 2012).
Summary:	Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.
Other form:	Print version: Metastasis research protocols. 2nd ed. New York : Humana Press : Springer, ©2012 9781617798535
Standard no.:	10.1007/978-1-61779-854-2

Table of Contents:

Basic immunocytochemistry for light microscopy / Susan A. Brooks
Lectin histochemistry to detect altered glycosylation in cells and tissues / Susan A. Brooks and Debbie M.S. Hall
Histopathological assessment of metastasis / Derek E. Roskell and Ian D. Buley
Immunocytochemical detection and characterisation of individual micrometastatic tumour cells / Stephan Braun and Klaus Pantel
The polymerase chain reaction / Hazel M. Welch
Sodium dodecyl sulphate-polyacrylamide denaturing gel electrophoresis and western blotting techniques / Christine Blancher and Rob McCormick
2-DE-based proteomics for the analysis of metastasis-associated proteins / Miriam Dwek and Diluka Peiris
Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography / Marta Toth, Anjum Sohail, and Rafael Fridman
Determination of cell-specific receptor binding using a combination of immunohistochemistry and in vitro autoradiography : relevance to therapeutic receptor targeting in cancer / Michael R. Dashwood and Marilena Loizidou
Fluorescence in situ hybridization for cancer-related studies / Lyndal Kearney and Janet Shipley
Using a quartz crystal microbalance biosensor for the study of metastasis markers on intact cells / Julien Saint-Guirons and Björn Ingemarsson
Cell separations by flow cytometry / Derek Davies
Detection of putative cancer stem cells of the side population phenotype in human tumor cell cultures / Matthias Christgen, Matthias Ballmaier, Ulrich Lehmann, and Hans Kreipe
Genes involved in the metastatic cascade of medullary thyroid tumours / Caroline Schreiber, Kirsten Vormbrock and Ulrike Ziebold
High-resolution quantitative methylation analysis of microRNA genes using PyrosequencingTM / Ulrich Lehmann, Cord Albat, and Hans Kreipe
RNAi technology to block the expression of molecules relevant to metastasis : the cell adhesion molecule CEACAM1 as an instructive example / Daniel Wicklein
Galectin-3 binding and metastasis / Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz
Lectin array-based strategies for identifying metastasis-associated changes in glycosylation / Simon Fry, Babak Afrough, Anthony Leathem, and Miriam Dwek.

Metastasis research protocols /

Similar Items