Protein NMR techniques /

Protein NMR techniques /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (xi, 487 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; 278 Methods in molecular biology (Clifton, N.J.) ; v. 278.
Subject:	Proteins -- Analysis. Nuclear magnetic resonance spectroscopy. Proteins -- analysis. Automation, Laboratory. Nuclear Magnetic Resonance, Biomolecular -- methods. Nuclear magnetic resonance spectroscopy. Proteins -- Analysis.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11137234

Hidden Bibliographic Details
Other authors / contributors:	Downing, A. Kristina (Anna Kristina)
ISBN:	1592598099 9781592598090 1588292460 9781588292469 128035996X 9781280359965 9786610359967 6610359962 9781617374494 1617374490 9781489939463 1489939466
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology"!series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules
Other form:	Print version: Protein NMR techniques. 2nd ed. Totowa, N.J. : Humana Press, ©2004 1588292460 1592598099
Standard no.:	10.1385/1592598099.

Description
Summary:	When I was asked to edit the second edition of Protein NMR Techniques, my first thought was that the time was ripe for a new edition. The past several years have seen a surge in the development of novel methods that are truly revolutionizing our ability to characterize biological macromolecules in terms of speed, accuracy, and size limitations. I was particularly excited at the prospect of making these techniques accessible to all NMR labs and for the opportunity to ask the experts to divulge their hints and tips and to write, practically, about the methods. I commissioned 19 chapters with wide scope for Protein NMR Techniques, and the volume has been organized with numerous themes in mind. Chapters 1 and 2 deal with recombinant protein expression using two organisms, E. coli and P. pastoris, that can produce high yields of isotopically labeled protein at a reasonable cost. Staying with the idea of isotopic labeling, Chapter 3 describes methods for perdeuteration and site-specific protonation and is the first of several chapters in the book that is relevant to studies of higher molecular weight systems. A different, but equally powerful, method that uses molecular biology to "edit" the spectrum of a large molecule using segmental labeling is presented in Chapter 4. Having successfully produced a high molecular weight target for study, the next logical step is data acquisition. Hence, the final chapter on this theme, Chapter 5, describes TROSY methods for stru- ural studies.
Physical Description:	1 online resource (xi, 487 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	1592598099 9781592598090 1588292460 9781588292469 128035996X 9781280359965 9786610359967 6610359962 9781617374494 1617374490 9781489939463 1489939466