Autoimmunity : methods and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (xiv, 431 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine, 1543-1894 ; 102 Methods in molecular medicine ; 102.
Subject:	Autoimmune diseases -- Laboratory manuals. Autoimmunity -- Laboratory manuals. Autoimmunity -- physiology Autoimmune Diseases -- physiopathology Models, Immunological T-Lymphocytes -- immunology Autoimmune diseases. Autoimmunity. Laboratory Manuals Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11137238

Hidden Bibliographic Details
Other authors / contributors:	Perl, Andras.
ISBN:	9781592598052 1588292312 9781588292315 1592598056 1280360003 9781280360008 9786610360000 6610360006 9781617374395
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Autoimmunity-which affects up to 10% of the world's population-can damage nearly every tissue or cell type of the human body with greatly debilitating or fatal results. In Autoimmunity: Methods and Protocols, expert medical researchers describe in step-by-step detail molecular techniques and in vitro/in vivo model systems for discovering the causes of autoimmunity, as well as how best it may be regulated. The methods to assess the immunological and biochemical pathways relevant for pathogenesis include identifying susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor, signal processing via protein kinases, identification and enumeration of antigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Additional protocols serve to establish the presence of and assess inflammatory arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, multiple sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and vitiligo. These methods can be used to assess the genetic, immunological, and biochemical parameters underlying spontaneous or exogenous antigen-induced diseases. The protocols follow the successful Methods in Molecular Medicine"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and easy to follow, Autoimmunity: Methods and Protocols offers today's investigators a cutting-edge collection of readily reproducible methods for establishing and interrogating human and animal models of autoimmune diseases
Other form:	Print version: Autoimmunity. Totowa, N.J. : Humana Press, ©2004 1588292312
Standard no.:	10.1385/1592598056.

Table of Contents:

Pathogenesis and spectrum of autoimmunity / Andras Perl
Models of pathogenesis in human autoimmune disorders
Mapping the systematic lupus erythematosus susceptibility genes / Swapan K. Nath, John B. Harley, and R. Hal Scofield
T cell signaling abnormalities in human systemic lupus erythematosus / Madhusoodana P. Nambiar, Sandeep Krishnan, and George Tsokos
TCR chain abnormalities in human systemic lupus erythematosus / Madhusoodana P. Nambiar [and others]
Protein kinase A and signal transduction in T lymphocytes : biochemical and molecular methods / Islam U. Khan and Gary M. Kammer
Apoptosis and mitochondrial dysfunction in lymphocytes of patients with systemic lupus erythematosus / Andras Perl [and others]
Methods for inducing apoptosis / Kathryn M. Roberts, Antony Rosen, and Livia A. Casciola-Rosen
Measurement of cytokines in autoimmune disease / Kyriakos A. Kirou, Christina Lee, and Mary K. Crow
Evaluation of autoimmunity to transaldolase in multiple sclerosis / Brian Niland and Andras Perl
Animal models of autoimmunity
Animal models for autoimmune myocarditis and autoimmune thyroiditis / Daniela Ciháková [and others]
Animal models of insulin-dependent diabetes mellitus / Edwin Liu [and others].
Generation, maintenance, and adoptive transfer of diabetogenic T cell lines/clones from the nonobese diabetic mouse / Martha J. Milton [and others]
Experimental use of murine lupus models / Stanford L. Peng
The anti-DNA knockin model of systemic autoimmunity induced by the chronic graft-vs-host reaction / Robert Eisenberg and Arpita Choudhury
Murine models of lupus induced by hypomethylated T cells / Bruce Richardson, Donna Ray, and Raymond Yung
The mouse model of collagen-induced arthritis / David D. Brand, Andrew H. Kang, and Edward F. Rosloniec
Proteoglycan aggrecan-induced arthritis: a murine autoimmune model of rheumatoid arthritis / Tibor T. Glant
Methods for induction and analysis of mouse models of multiple
Sclerosis : experimental autoimmune encephalomyelitis and Theiler's virus-induced demyelinating disease / Kevin G. Fuller [and others]
Experimental autoimmune encephalitomyelitis / Praveen Rao and Benjamin M. Segal
Animal models of scleroderma / Gabriella Lakos, Shinsuke Takagawa, and John Varga
Rodent models of experimental autoimmune uveitis / Rajeev K. Agarwal and Rachel R. Caspi
Autoimmune depigmentation following sensitization to melanoma antigens / Arthur A. Hurwitz and Qingyong Ji.

Autoimmunity : methods and protocols /

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