Germ cell protocols. Part 2, Molecular embryo analysis, live imaging, transgenesis, and cloning /

Germ cell protocols. Part 2, Molecular embryo analysis, live imaging, transgenesis, and cloning /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (1 volume) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology ; v. 254, pt. 2 Methods in molecular biology (Clifton, N.J.) ; volume 254, pt. 2.
Subject:	Veterinary embryology -- Research -- Technique. Germ cells -- Research -- Technique. Cytological Techniques. Germ Cells. Genetic Techniques.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11137534

Hidden Bibliographic Details
Varying Form of Title:	Molecular embryo analysis, live imaging, transgenesis, and cloning
Other authors / contributors:	Schatten, Heide.
ISBN:	1592597416 9781592597413 9781588292575 1588292576 1588292576
Notes:	Includes bibliographical references and indexes. Print version record.
Summary:	Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, the imaging of cell structures, and the cryopreservation of germ cells. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. There are also methods for PCR-based cloning, differentiating embryonic stem cells into embryonic bodies, live imaging of mammalian embryos using multiphoton microscopy, and the cloning of pig, cattle, rabbit, and mouse oocytes. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying earlier volume, Sperm and Oocyte Analysis, focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.
Other form:	Print version: Germ cell protocols. Volume 2, Molecular embryo analysis, live imaging, transgenesis, and cloning. Totowa, N.J. : Humana Press, ©2004 1588291219 1588292576
Standard no.:	10.1385/1592597416

Description
Summary:	The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits st- ies of fertility and infertility in mammals including humans. External in vitro fertilization, now a common technique in assisted fertilization has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex that carry to- potency, an ability to develop into an entire new organism. The two volumes on germ cells combine techniques in a variety of different systems and have selected those systems that have provided landmarks in advancing our kno- edge on germ cells.
Physical Description:	1 online resource (1 volume) : illustrations (some color)
Bibliography:	Includes bibliographical references and indexes.
ISBN:	1592597416 9781592597413 9781588292575 1588292576