GTPases regulating membrane targeting and fusion /

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Bibliographic Details
Imprint:	Amsterdam ; Boston : Elsevier Academic Press, ©2005.
Description:	1 online resource (xliii, 863 pages, [28] pages of plates) : illustrations (some color).
Language:	English
Series:	Methods in enzymology, 0076-6879 ; v. 403 Methods in enzymology ; v. 403.
Subject:	Cellular control mechanisms. Enzymes. Membrane fusion. Membranes (Biology) Cellular control mechanisms. Enzymes. Membrane fusion. Membranes (Biology) Enzymes [MESH] Membrane Fusion [MESH] Membranes [MESH] GTP Phosphohydrolases [MESH] Periodicals Electronic journals. Electronic books.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11141412

Hidden Bibliographic Details
Other authors / contributors:	Balch, William Edward, 1949- Der, Channing J. Hall, A. (Alan)
ISBN:	0121828085 9780121828080
Notes:	Includes bibliographical references and index. Print version record.
Summary:	Rab GTPases now comprise a family of>63 members. They are emerging as the key hub element controlling the membrane architecture of eukaryotic cells. They are intimately involved in vesicle targeting and fusion in both the endocytic and exocytic pathways and direct the assembly and disassembly of protein complexes that include regulators (GEFs and GAPs), effectors (tethers/motors) and fusion components (SNAREs) that control membrane targeting and fusion. During the last 3 years the field has virtually exploded with the identification and characterization of many new Rab proteins and their effectors. Our understanding of how Rab GTPases control membrane function remains at its infancy. This volume of MIE provides a wealth of new concepts, approaches and tools to study Rab proteins in the test tube and in living cells that will be of strong benefit to both established laboratories and new investigators in the field to elucidate Rab GTPase function in cellular development, differentiation and proliferation. * Comprehensive overview of Rab GTPase phylogeny and systems biology * Identification and characterization of Rab GEFs, GAPs and effectors * General methodologies to study Rab GTPase function in vitro and in vivo using biochemical, molecular and microscopy approaches.

Table of Contents:

Contributors to Volume 403
Preface
Volumes in Series
1. Exploring Trafficking GTPase Function by mRNA Expression Profiling: Use of the SymAtlas Web-Application and the Membrome Datasets
2. Use of Search Algorithms to Define Specificity in Rab GTPase Domain Function
3. Application of Phylogenetic Algorithms to Assess Rab Functional Relationships
4. Application of Protein Semisynthesis for the Construction of Functionalized Posttranslationally Modified Rab GTPases
5. Assay and Functional Properties of PrBP(PDE[delta]), a Prenyl-Binding Protein Interacting with Multiple Partners
6. Functional Assays for the Investigation of the Role of Rab GTPase Effectors in Dense Core Granule Release
7. Analysis of Rab1 Recruitment to Vacuoles Containing Legionella pneumophila
8. Reconstitution of Rab4-Dependent Vesicle Formation In Vitro
9. Assay of Rab4-Dependent Trafficking on Microtubules
10. CD2AP, Rabip4, and Rabip4': Analysis of Interaction with Rab4a and Regulation of Endosomes Morphology
11. Visualization of Rab5 Activity in Living Cells Using FRET Microscopy
12. Selection and Application of Recombinant Antibodies as Sensors of Rab Protein Conformation
13. Analysis of Rab Protein Function in Neurotransmitter Receptor Trafficking at Hippocampal Synapses
14. Use of Rab GTPases to Study Lipid Trafficking in Normal and Sphingolipid Storage Disease Fibroblasts
15. Assay of Rab13 in Regulating Epithelial Tight Junction Assembly
16. Tyrosine Phosphorylation of Rab Proteins
17. Assay of Rab25 Function in Ovarian and Breast Cancers
18. Functional Analysis of Rab27a Effector Granuphilin in Insulin Exocytosis
19. Assays for Functional Properties of Rab34 in Macropinosome Formation
20. Fluorescent Microscopy-Based Assays to Study the Role of Rab22a in Clathrin-Independent Endocytosis
21. Purification and Properties of Rab3 GEP (DENN/MADD)
22. Biochemical Characterization of Alsin, a Rab5 and Rac1 Guanine Nucleotide Exchange Factor
23. Purification and Analysis of RIN Family-Novel Rab5 GEFs
24. Purification and Functional Properties of a Rab8-Specific GEF (Rabin3) in Action Remodeling and Polarized Transport
25. Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella-Containing Phagosomes
26. Purification and Functional Analyses of ALS2 and its Homologue
27. Polycistronic Expression and Purification of the ESCRT-II Endosomal Trafficking Complex
28. Analysis and Properties of the Yeast YIP1 Family of Ypt-Interacting Proteins
29. Use of Hsp90 Inhibitors to Disrupt GDI-Dependent Rab Recycling
30. Purification and Properties of Yip3/PRA1 as a Rab GDI Displacement Factor
31. Purification and Analysis of TIP47 Function in Rab9-Dependent Mannose 6-Phosphate Receptor Trafficking
32. Capture of the Small GTPase Rab5 by GDI: Regulation by p38 MAP Kinase
33. Rab2 Purification and Interaction with Protein Kinase C [iota]/[lambda] and Glyceraldehyde-3-Phosphate Dehydrogenase
34. Purification and Functional Interactions of GRASP55 with Rab2
35. Purification and Properties of Rabconnectin-3
36. Physical and Functional Interaction of Noc2/Rab3 in Exocytosis
37. Functional Analysis of Slac2-a/Melanophilin as a Linker Protein between Rab27A and Myosin Va in Melanosome Transport
38. Identification and Biochemical Analysis of Slac2-c/MyRIP as a Rab27A-, Myosin Va/VIIa-, and Actin-Binding Protein
39. Analysis of the Role of Rab27 Effector Slp4-a/Granuphilin-a in Dense-Core Vesicle Exocytosis
40. Assay and Functional Interactions of Rim2 with Rab3
41. Assay of the Rab-Binding Specificity of Rabphilin and Noc2: Target Molecules for Rab27
42. Functional Properties of the Rab-Binding Domain of Rab-Coupling Protein
43. Purification and Functional Properties of Rab11-FIP2
44. Purification and Functional Properties of Rab11-FIP3
45. Class I FIPs, Rab11-Binding Proteins That Regulate Endocytic Sorting and Recycling
46. Expression and Properties of the Rab4, Rabaptin-5[alpha], AP-1 Complex in Endosomal Recycling
47. Measurement of the Interaction of the p85[alpha] Subunit of Phosphatidylinositol 3-Kinase with Rab5
48. Assay and Stimulation of the Rab5 GTPase by the p85[alpha] Subunit of Phosphatidylinositol 3-Kinase
49. Ubiquitin Regulation of the Rab5 Family GEF Vps9p
50. Analysis of the Interaction between GGA1 GAT Domain and Rabaptin-5
51. Purification and Properties of Rab6 Interacting Proteins
52. Affinity Purification of Ypt6 Effectors and Identification of TMF/ARA160 as a Rab6 Interactor
53. Assay and Properties of Rab6 Interaction with Dynein-Dynactin Complexes
54. Assay and Functional Properties of Rabkinesin-6/Rab6-KIFL/MKlp2 in Cytokinesis
55. Interaction and Functional Analyses of Human VPS34/p150 Phosphatidylinositol 3-Kinase Complex with Rab7
56. Functional Analyses and Interaction of the XAPC7 Proteasome Subunit with Rab7
57. Expression, Assay, and Functional Properties of RILP
58. Assay and Functional Properties of Rab34 Interaction with RILP in Lysosome Morphogenesis
59. Rabring7: A Target Protein for Rab7 Small G Protein
60. Analysis of Potential Binding of the Recombinant Rab9 Effector p40 to Phosphoinositide-Enriched Synthetic Liposomes
61. Assessment of Rab11-FIP2 Interacting Proteins In Vitro
62. Interactions of Myosin Vb with Rab11 Family Members and Cargoes Traversing the Plasma Membrane Recycling System
63. Properties of Rab13 Interaction with Protein Kinase A
64. Functional Properties of Rab15 Effector Protein in Endocytic Recycling
65. Assays for Interaction between Rab7 and Oxysterol Binding Protein Related Protein 1L (ORP1L)
66. Characterization of Rab23, a Negative Regulator of Sonic Hedgehog Signaling
67. Purification and Functional Analysis of a Rab27 Effector Munc13-4 Using a Semiintact Platelet Dense-Granule Secretion Assay
68. Analysis of hVps34/hVps15 Interactions with Rab5 In Vivo and In Vitro
69. Purification and Functional Properties of Prenylated Rab Acceptor 2
Author Index
Subject Index

GTPases regulating membrane targeting and fusion /

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