Human papillomaviruses : methods and protocols /
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Imprint: | Totowa, N.J. : Humana Press, ©2005. |
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Description: | 1 online resource (xv, 496 pages) : illustrations |
Language: | English |
Series: | Methods in molecular medicine ; 119 Methods in molecular medicine ; 119. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11150202 |
Table of Contents:
- Preface
- Contributors
- 1. Identification of New Papillomavirus Types
- 2. Identification of HPV Variants
- 3. Histochemical Analysis of Cutaneous HPV-Associated Lesions
- 4. Histological Analysis of Cervical Intraepithelial Neoplasia
- 5. Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections
- 6. Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
- 7. Analysis of p16 INK4a and Integrated HPV Genomes as Progression Markers
- 8. Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
- 9. HPV DNA Detection and Typing in Cervical Scrapes
- 10. HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions
- 11. Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies
- 12. Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic "Raft" Cultures
- 13. Differentiation of HPV-Containing Cells Using Organotypic "Raft" Culture or Methylcellulose
- 14. Propagation of Infectious, High-Risk HPV in Organotypic "Raft" Culture
- 15. Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic "Raft" Cultures
- 16. The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
- 17. The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
- 18. Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
- 19. Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
- 20. Analysis of the Regulation of Viral Transcription
- 21. Analysis of HPV Transcription by RPA
- 22. Analysis of Regulatory Motifs Within HPV Transcripts
- 23. Detection of HPV Transcripts by Nested RT-PCR
- 24. Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
- 25. Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCR
- 26. Analysis of E7/Rb Associations
- 27. Transformation Assays for HPV Oncoproteins
- 28. Analysis of Adeno-Associated Virus and HPV Interaction
- 29. In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
- 30. Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
- 31. Codon Optimization of Papillomavirus Genes
- 32. Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays
- 33. Generation and Application of HPV Pseudovirions Using Vaccinia Virus
- Index