Human papillomaviruses : methods and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2005.
Description:	1 online resource (xv, 496 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine ; 119 Methods in molecular medicine ; 119.
Subject:	Papillomavirus diseases -- Laboratory manuals. Papillomaviruses -- Laboratory manuals. Papillomavirus, Human -- genetics Papillomavirus, Human -- pathogenicity Uterine Cervical Neoplasms -- virology. Papillomavirus diseases. Papillomaviruses. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11150202

Hidden Bibliographic Details
Other authors / contributors:	Davy, Clare. Doorbar, John.
ISBN:	1588293734 9781588293732 9781592599820 1592599826
Notes:	Includes bibliographical references and index.
Summary:	Human papillomaviruses (HPV) are an important class of pathogens responsible for a variety of diseases, including cervical cancer, the second most commonly found female cancer worldwide. In Human Papillomaviruses: Methods and Protocols, leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of this formidable virus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Human Papillomaviruses: Methods and Protocols offers both novice and experienced investigators a set of highly successful analytical tools for unlocking the secrets of the human papillomaviruses and their pathologies.
Other form:	Print version: Human papillomaviruses. Totowa, N.J. : Humana Press, ©2005 1588293734 1592599826

Table of Contents:

Preface
Contributors
1. Identification of New Papillomavirus Types
2. Identification of HPV Variants
3. Histochemical Analysis of Cutaneous HPV-Associated Lesions
4. Histological Analysis of Cervical Intraepithelial Neoplasia
5. Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections
6. Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
7. Analysis of p16 INK4a and Integrated HPV Genomes as Progression Markers
8. Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
9. HPV DNA Detection and Typing in Cervical Scrapes
10. HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions
11. Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies
12. Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic "Raft" Cultures
13. Differentiation of HPV-Containing Cells Using Organotypic "Raft" Culture or Methylcellulose
14. Propagation of Infectious, High-Risk HPV in Organotypic "Raft" Culture
15. Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic "Raft" Cultures
16. The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
17. The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
18. Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
19. Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
20. Analysis of the Regulation of Viral Transcription
21. Analysis of HPV Transcription by RPA
22. Analysis of Regulatory Motifs Within HPV Transcripts
23. Detection of HPV Transcripts by Nested RT-PCR
24. Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
25. Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCR
26. Analysis of E7/Rb Associations
27. Transformation Assays for HPV Oncoproteins
28. Analysis of Adeno-Associated Virus and HPV Interaction
29. In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
30. Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
31. Codon Optimization of Papillomavirus Genes
32. Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays
33. Generation and Application of HPV Pseudovirions Using Vaccinia Virus
Index

Human papillomaviruses : methods and protocols /

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