Gene expression systems : using nature for the art of expression /

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Bibliographic Details
Imprint:San Diego : Academic Press, ©1999.
Description:1 online resource (xii, 480 pages) : illustrations
Language:English
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11153533
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Other authors / contributors:Fernandez, Joseph M.
Hoeffler, James P.
ISBN:9780122538407
0122538404
9780080532356
0080532357
1281054186
9781281054180
9786611054182
6611054189
Notes:Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
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Print version record.
Summary:Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method.
Other form:Print version: Gene expression systems. San Diego : Academic Press, ©1999 0122538404 9780122538407
Table of Contents:
  • Contributors
  • Introduction: The Art of Expression
  • Purpose of This Book
  • Selecting a Suitable Expression System: Considerations
  • Genomics and the Future of Protein Expression Systems
  • Prokaryotic Expression Systems: Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse
  • Expression Vectors Employing the trc Promoter
  • Bacillus Expression: A Gram Positive Model
  • araB Expression System in Escherichia coli
  • Eukaryotic Expression Systems: Adenoviral Vectors for Protein Expression
  • Expression in the Methalotrophic Yeast Pichia pastoris
  • Recombinant Protein Expression in Pichia methanolica
  • Cytomegalovirus Promoter for Expression in Mammalian Cells
  • Inducible Mammalian Expression Systems
  • Protein Expression in Mammalian Cells Using Sindbis Virus
  • Expression in Insect Systems: Drosophila S2 System for Heterologous Gene Expression
  • Baculovirus Expression Vector System
  • Transgenic Expression: Recombinant Protein Expression in Transgenic Mice
  • Expression of Recombinant Proteins in the Milk of Transgenic Animals
  • Recombinant Protein Expression in Plants
  • Index