Lentivirus gene engineering protocols /

Lentivirus gene engineering protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, 2003.
Description:	1 online resource (xi, 314 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; v. 229 Methods in molecular biology (Clifton, N.J.) ; v. 229.
Subject:	Genetic engineering. Lentiviruses. Genetic vectors. Lentivirus -- genetics. Gene Targeting -- methods. Gene Transfer Techniques. Genetic Engineering -- methods. Genetic Vectors -- biosynthesis. Genetic engineering. Genetic vectors. Lentiviruses. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11154627

Hidden Bibliographic Details
Other authors / contributors:	Federico, Maurizio.
ISBN:	9781592593934 1588290913 9781588290915 1592593933 1280842687 9781280842689 9786610842681 661084268X 9781617373510
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.
Other form:	Print version: Lentivirus gene engineering protocols. Totowa, N.J. : Humana Press, 2003 1588290913 9781588290915
Standard no.:	10.1385/1592593933.

Description
Summary:	Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.
Physical Description:	1 online resource (xi, 314 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	9781592593934 1588290913 9781588290915 1592593933 1280842687 9781280842689 9786610842681 661084268X 9781617373510