Meningococcal vaccines : methods and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2001.
Description:1 online resource (xvii, 421 pages) : illustrations
Language:English
Series:Methods in molecular medicine ; 66
Methods in molecular medicine ; 66.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154634
Hidden Bibliographic Details
Other uniform titles:Meningococcal disease.
Other authors / contributors:Pollard, Andrew J.
Maiden, Martin C. J.
ISBN:0896038017
9780896038011
9781592591480
1592591485
1280821132
9781280821134
9786610821136
6610821135
Notes:Companion volume to: Meningococal disease.
Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:The development of a comprehensive vaccine against Neisseria meningitidis, the causative agent of meningococcal disease, has remained elusive because of bacterial diversity and immunologic evasion. In Meningococcal Vaccines: Methods and Protocols, Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. They also present as a test case the recent implementation of a new meningococcal vaccine in the United Kingdom. A companion volume, Meningococcal Disease: Methods and Protocols, contains detailed methods for diagnostic microbiology, bacterial characterization, epidemiology, host-pathogen interactions, and clinical studies. Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus and to develop, evaluate, and implement successful new meningococcal vaccines.
Other form:Print version: Meningococcal vaccines. Totowa, N.J. : Humana Press, ©2001 0896038017
Online version: Meningococcal vaccines. Totowa, N.J. : Humana Press, ©2001
Standard no.:10.1385/1592591485.
Table of Contents:
  • Foreword
  • Preface
  • Contributors
  • 1. Meningococcal Vaccines and Vaccine Developments
  • 2. Immune Response and Host-Pathogen Interactions
  • 3. Purification of Capsular Polysaccharide
  • 4. Protein-Polysaccharide Conjugation
  • 5. N-Propionylation
  • 6. Outer Membrane Protein Purification
  • 7. Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease
  • 8. Methods for Manipulation of Transferrin-Binding Proteins
  • 9. Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins
  • 10. Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics
  • 11. Construction of porA Mutants
  • 12. Construction of LPS Mutants
  • 13. Recombinant Proteins in Vaccine Development
  • 14. Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries
  • 15. Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity
  • 16. Early Experiences with Nasal Vaccines against Meningococcal Disease
  • 17. Animal Models for Meningococcal Disease
  • 18. Determination of Antibody Responses to Meningococcal Antigens by ELISA
  • 19. Immunoblot Analysis of Sera from Patients and Vaccinees
  • 20. Serogroup B and C Serum Bactericidal Assays
  • 21. A Modified ELISA for Measurement of High-Avidity lgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers
  • 22. Whole-Blood Model
  • 23. Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
  • 24. T-Cell Responses Against Meningococcal Antigens
  • 25. CD4 T-Cell Epitope Mapping
  • 26. Epitope Mapping
  • 27. Meningococcal Vaccine Trials
  • 28. The Introduction of Group C Conjugate Meningococcal Vaccine into the UK
  • Index