Calpain methods and protocols /

Calpain methods and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2000.
Description:1 online resource (xviii, 343 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 144
Methods in molecular biology (Clifton, N.J.) ; v. 144.
Subject:
Calpain -- Laboratory manuals.
Cysteine proteinases.
Hydrolases.
Enzymes.
Calpain.
Cysteine Endopeptidases
Cysteine Proteases
Endopeptidases
Peptide Hydrolases
Hydrolases
Enzymes
Enzymes and Coenzymes
Chemicals and Drugs
Calpain
Hydrolases
Enzymes
Cysteine proteinases
Calpain
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154667
Hidden Bibliographic Details
Other authors / contributors:Elce, John S.
ISBN:9781592590506
0896036324
9780896036321
1592590500
128082042X
9781280820427
9786610820429
6610820422
9781617371059
Notes:Includes bibliographical references and index.
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of æ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains.
Other form:Print version: Calpain methods and protocols. Totowa, N.J. : Humana Press, ©2000 0896036324 9780896036321
Standard no.:10.1385/1592590500
Table of Contents:
  • pt. I. Purification of Components of the Calpain System. 1. Purification of [mu]-Calpain, m-Calpain, and Calpastatin from Animal Tissues / Valery F. Thompson and Darrel E. Goll. 2. A Simple Protocol for Separation and Assay of [mu]-Calpain, m-Calpain, and Calpastatin from Small Tissue Samples / Jan-Olof Karlsson. 3. Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms From Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography / Elsa J.B. Murray, Keyvan Behnam and Mario S. Grisanti / [and others]. 4. Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose / Dorothy E. Croall. 5. Affinity Purification of [mu]-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump: Some Studies on Erythrocyte [mu]-Calpain / Maurizio Molinari and Ernesto Carafoli. 6. Expression of m-Calpain in Escherichia coli / John S. Elce.

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