Apoptosis methods in pharmacology and toxicology : approaches to measurement and quantification /

Apoptosis methods in pharmacology and toxicology : approaches to measurement and quantification /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2002.
Description:1 online resource (xiv, 156 pages) : illustrations
Language:English
Series:Methods in pharmacology and toxicology, 1940-6053
Methods in pharmacology and toxicology.
Subject:
Apoptosis -- Research -- Methodology.
Apoptosis -- physiology.
Cytological Techniques.
Apoptosis -- Research -- Methodology.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154709
Hidden Bibliographic Details
Other authors / contributors:Davis, Myrtle A.
ISBN:9781592592791
1592592791
1280830646
9781280830648
9786610830640
6610830649
0896038904
9780896038905
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Apoptosis, or programmed cell death, is a widespread cellular process that regulates numerous important biological events ranging from the metamorphosis of the tadpole tail to the elimination of surplus brain cells in the formation of proper synaptic connections. In Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification, Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful in toxicology and pharmacology research. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high-throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems. Presented by authors well-versed in the technical problems and challenges in analyzing apoptosis, each method explicates its successful use, describes its limitations, and shows how it may be applied in large-scale screening operations. Concise and eminently practical, Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification offers pharmacologists, toxicologists, pathologists, and many other biomedical scientists today's gold standard reference source for methods that definitively identify and accurately quantify apoptosis.
Other form:Print version: Apoptosis methods in pharmacology and toxicology. Totowa, N.J. : Humana Press, ©2002 0896038904
Standard no.:10.1385/1592592791.
Table of Contents:
  • Apoptosis Methods in Pharmacology and Toxicology
  • Series Foreword
  • Preface
  • Contents
  • Contributors
  • Introduction
  • Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death
  • Analysis of Apoptosis by Laser-Scanning Cytometry
  • Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections
  • DNA Microarrays
  • Microarray Analysis of Apoptosis
  • ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases
  • Index

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