Protein misfolding and disease : principles and protocols /

Protein misfolding and disease : principles and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2003.
Description:1 online resource (xv, 318 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 232
Methods in molecular biology (Clifton, N.J.) ; v. 232.
Subject:
Proteins -- Pathophysiology -- Laboratory manuals.
Protein folding -- Laboratory manuals.
Proteins -- Conformation.
Protein Folding
Protein Conformation
Proteins -- Conformation
Protein folding
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154987
Hidden Bibliographic Details
Other authors / contributors:Bross, Peter.
Gregersen, Niels.
ISBN:1592593941
9781592593941
1588290654
9781588290656
Notes:Includes bibliographical references and index.
Print version record.
Summary:It has now become clear that a large number of diseases with very different pathologies share a common framework of protein misfolding, accompanied by degradation and/or aggregation of the misfolded proteins. In Protein Misfolding and Disease: Principles and Protocols, notable experts in conformational disease review the latest thinking about the molecular processes underlying these diseases and describe cutting-edge biochemical, genomic, cellular, and chemical laboratory techniques for studying their genesis and pathologies. The authors apply their carefully refined methods to a variety of metabolic and neurodegenerative disorders, as well as to the aging process. The techniques presented are broadly applicable in many diverse disease contexts and may be used in both diagnosis and detailed research on new treatment strategies. Each tried and proven protocol includes insightful background notes, lists of required equipment and reagents, step-by-step instructions, and tips on troubleshooting and on how to avoid known pitfalls. Comprehensive and cutting-edge, Protein Misfolding and Disease: Principles and Protocols offers both novice and experienced researchers a solid theoretical grounding in conformational disease and a remarkable set of analytical methodologies for uncovering its characteristics and generating new therapeutic approaches.
Other form:Print version: Protein misfolding and disease. Totowa, N.J. : Humana Press, ©2003 1588290654 9781588290656
Table of Contents:
  • Preface
  • Contributors
  • I. General Concepts and Models
  • 1. Protein Misfolding, Aggregation, and Degradation in Disease
  • 2. Basic Introduction to In Vivo Protein Folding and Its Defects
  • 3. Cystic Fibrosis: Premature Degradation of Mutant Proteins as a Molecular Disease Mechanism
  • 4. [alpha]1-Antitrypsin Deficiency: Liver Disease Associated With Retention of a Mutant Secretory Glycoprotein in the Endoplasmic Reticulum
  • 5. Parkinson's Disease: [alpha]-Synuclein and Parkin in Protein Aggregation and the Reversal of Unfolded Protein Stress
  • 6. Aberrant Protein Folding as the Molecular Basis of Cancer
  • 7. Yeast as a Model System to Investigate Protein Conformational Diseases
  • II. General Methods
  • 8. Expression of Recombinant Proteins: An Introduction
  • 9. Expression in E. coli Systems
  • 10. Protein Expression in Yeasts
  • 11. Site-Directed Mutagenesis
  • 12. Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation
  • III. Techniques in Conformational Disease Research
  • 13. Detection of Aggregates and Protein Inclusions by Staining of Tissues
  • 14. Study of Mutant Proteins With Folding Defects in Cultured Patient Cells
  • 15. E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase
  • 16. Characterization of Overexpressed Mutant Proteins in Mammalian Cells
  • 17. Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants
  • 18. Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells
  • 19. Application of Chemical Chaperones to the Rescue of Folding Defects
  • 20. Analysis of Defective Subunit Interactions Using the Two-Hybrid System
  • 21. Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol
  • 22. Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes
  • 23. Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria
  • 24. Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells
  • Index

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