Molecular diagnosis of infectious diseases /

Molecular diagnosis of infectious diseases /

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Bibliographic Details
Edition:2nd ed.
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (xv, 464 pages) : illustrations
Language:English
Series:Methods in molecular medicine ; 94
Methods in molecular medicine ; 94.
Subject:
Communicable diseases -- Molecular diagnosis -- Laboratory manuals.
Communicable Diseases -- diagnosis.
Biological Assay.
Communicable Diseases -- genetics.
Immunologic Tests.
Recombinant Proteins.
Emerging infectious diseases.
Medicine.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11155049
Hidden Bibliographic Details
Other authors / contributors:Decker, Jochen.
Reischl, Udo.
ISBN:9781592596799
1588292215
9781588292216
1592596797
1280831545
9781280831546
9786610831548
6610831548
9781617374302
Notes:Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.
Other form:Print version: Molecular diagnosis of infectious diseases. 2nd ed. Totowa, N.J. : Humana Press, ©2004 1588292215 9781588292216
Standard no.:10.1385/1592596797
Table of Contents:
  • Foreword
  • Preface
  • Contributors
  • Part I.. Protein-Based Identification of Antigens
  • 1. Proteomic Approaches to Antigen Discovery
  • 2. Immunoproteomics
  • 3. Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
  • Part II.. DNA-Based Identification of Antigens
  • 4. Representational Difference Analysis of cDNA
  • 5. Microarray Data Analysis and Mining
  • 6. Expression Cloning
  • Part III.. Identification of Epitopes and Immunomodulatory Components
  • 7. Determination of Epitopes by Mass Spectrometry
  • 8. Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
  • 9. Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
  • 10. Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
  • Part IV.. Expression of Recombinant Proteins
  • 11. High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
  • 12. Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
  • 13. Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
  • Part V.. Purification, Modification, and Renaturation of Recombinant Proteins
  • 14. Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
  • 15. Purification of Recombinant Proteins with High Isoelectric Points
  • 16. Refolding of Inclusion Body Proteins
  • 17. Small-Molecule-Protein Conjugation Procedures
  • Part VI.. Characterization of Recombinant Proteins
  • 18. Structural Characterization of Proteins and Peptides
  • 19. Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
  • 20. Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
  • Part VII.. Evaluation of Recombinant Proteins in Immunological Test Systems
  • 21. Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
  • 22. Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
  • 23. Basic Problems of Serological Laboratory Diagnosis
  • 24. Molecular Diagnostics Resources on the Internet
  • Part VIII.. Recombinant Receptor Molecules
  • 25. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
  • Index

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