Molecular motors : methods and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2007.
Description:	1 online resource (xi, 247 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; 392 Methods in molecular biology (Clifton, N.J.) ; v. 392.
Subject:	Molecular biology. Biochemistry. Molecular Motor Proteins. Biochemistry. Molecular biology. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11156740

Hidden Bibliographic Details
Other authors / contributors:	Sperry, Ann O.
ISBN:	1597454907 9781597454902 1588296652 9781588296658 1588296652 9781588296658
Digital file characteristics:	text file
Notes:	Includes bibliographical references and index. Print version record.
Summary:	Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.
Other form:	Print version: Molecular motors. Totowa, N.J. : Humana Press, ©2007 9781588296658
Standard no.:	9781588296658 10.1007/978-1-59745-490-2 9786610971992

Table of Contents:

Equilibrium binding of proteins to F-actin / Joseph M. Chalovich
Analysis of calcium/calmodulin regulation of a plant kinesin using co-sedimentation and ATPase assays / Anireddy S.N. Reddy
In vitro and in vivo analysis of microtubule-destabilizing kinesins / Jason Stumpff [and others]
Approaches to kinesin-1 phosphorylation / Gerardo Morfini, Gustavo Pigino, and Scott T. Brady
Protein modification to probe intradynein interactions in in vivo redox state / Ken-ichi Wakabayashi, Miho Sakato, and Stephen M. King
Methods to study the interactions of the dynein light chains and intermediate chains / Kevin W.-H. Lo and K. Kevin Pfister
Identification of motor protein cargo by yeast 2-hybrid and affinity approaches / Yuguo Zhang [and others]
In situ binding assay to detect myosin-1c interactions with hair-cell proteins / Kelli R. Phillips and Janet L. Cyr
Ultrastructural analysis of kenesin-related motor proteins during spermatogenesis / Wan-Xi Yang
In vitro motility system to study the role of motor proteins in receptor-ligand sorting / John W. Murray and Allan W. Wolkoff
Enrichment and disassembly of ectoplasmic specializations in the rat testis / Julian A. Guttman, Kuljeet S. Vaid, and A. Wayne Vogl
Single-molecule observation of rotation of F1-ATPase through microbeads / Takayuki Nichizaka, Kana Mizutani, and Tomoko Masaike
The use of FRET in the analysis of motor protein structure / Andrzej A. Kasprzak
Structure determination of the motor domain of yeast kinesin Kar3 by X-ray crystallography / Hee-Won Park
High-resolution structural analysis of the kinesin-microtubule complex by electron cryo-microscopy / Keiko Hirose and Linda A. Amos
Chemical-genetic inhibition of sensitized mutant unconventional myosins / Ryan L. Karcher [and others].

Molecular motors : methods and protocols /

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