Pichia protocols /

Pichia protocols /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, N.J. : Humana Press, 2007.
Description:	1 online resource (xi, 268 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; 389 Methods in molecular biology (Clifton, N.J.) ; v. 389.
Subject:	Pichia pastoris. Genetic vectors. Yeast fungi -- Biotechnology. Pichia -- genetics. Recombinant Proteins. Genetic vectors. Pichia pastoris. Yeast fungi -- Biotechnology. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11156742

Hidden Bibliographic Details
Other authors / contributors:	Cregg, James M.
ISBN:	9781588294296 1588294293 1597454567 9781597454568
Digital file characteristics:	text file PDF
Notes:	Previous edition: 1998. Title from title screen (viewed November 20, 2007). Includes bibliographical references and index. English.
Summary:	The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system.
Other form:	Print version: Cregg, James M. Pichia protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2007 9781588294296 1588294293
Standard no.:	10.1007/978-1-59745-456-8

Description
Summary:	Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.
Item Description:	Previous edition: 1998. Title from title screen (viewed November 20, 2007).
Physical Description:	1 online resource (xi, 268 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	9781588294296 1588294293 1597454567 9781597454568