Pichia protocols /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, N.J. : Humana Press, 2007.
Description:	1 online resource (xi, 268 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; 389 Methods in molecular biology (Clifton, N.J.) ; v. 389.
Subject:	Pichia pastoris. Genetic vectors. Yeast fungi -- Biotechnology. Pichia -- genetics. Recombinant Proteins. Genetic vectors. Pichia pastoris. Yeast fungi -- Biotechnology. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11156742

Hidden Bibliographic Details
Other authors / contributors:	Cregg, James M.
ISBN:	9781588294296 1588294293 1597454567 9781597454568
Digital file characteristics:	text file PDF
Notes:	Previous edition: 1998. Title from title screen (viewed November 20, 2007). Includes bibliographical references and index. English.
Summary:	The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system.
Other form:	Print version: Cregg, James M. Pichia protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2007 9781588294296 1588294293
Standard no.:	10.1007/978-1-59745-456-8

Table of Contents:

Preface
Contributors
1. Introduction: Distinction Between Pichia pastoris and Other Expression Systems
2. Vectors and Strains for Expression
3. DNA-Mediated Transformation
4. Rational Design and Optimization of Fed-Batch and Continuous Fermentations
5. Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1)
6. Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C
7. Rapid Screening of Chromatography Resins for the Purification of Proteins
8. Characterization of O-Linked Saccharides on Glycoproteins
9. Modification of the N-Glycosylation Pathway to Produce Homogeneous Human-Like Glycans Using GlycoSwitch Plasmids
10. N-Linked Glycan Characterization of Heterologous Proteins
11. Heavy Labeling of Recombinant Proteins
12. Selenomethionine Labeling of Recombinant Proteins
13. Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains
14. Classical Genetics
15. Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration
16. Characterization of Protein-Protein Interactions: Application to the Understanding of Peroxisome Biogenesis
17. Localization of Proteins and Organelles Using Fluorescence Microscopy
18. Fluorescence Microscopy and Thin-Section Electron Microscopy
Index

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