Adhesion protein protocols.

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Bibliographic Details
Edition:2nd ed. / edited by Amanda S. Coutts.
Imprint:Totowa, N.J. : Humana Press, ©2007.
Description:1 online resource (x, 234 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1064-3745 ; 370
Methods in molecular biology (Clifton, N.J.) ; v. 370.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11157685
Hidden Bibliographic Details
Other authors / contributors:Coutts, Amanda S.
ISBN:1588295338
9781588295330
9781597453530
1597453536
Notes:Includes bibliographical references and index.
Print version record.
Summary:The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Other form:Print version: Adhesion protein protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2007 1588295338
Standard no.:10.1007/978-1-59745-353-0

MARC

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250 |a 2nd ed. /  |b edited by Amanda S. Coutts. 
260 |a Totowa, N.J. :  |b Humana Press,  |c ©2007. 
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490 1 |a Methods in molecular biology,  |x 1064-3745 ;  |v 370 
504 |a Includes bibliographical references and index. 
505 0 |a Manipulation of cell-cell adhesion using bowtie-shaped microwells / Celeste M. Nelson, Wendy F. Liu, and Christopher S. Chen -- Separation of cell-cell adhesion complexes by differential centrifugation / Roger Vogelmann and W. James Nelson -- Analysis of neutrophil chemotaxis / Paul A. Nuzzi, Mary A. Lokuta, and Anna Huttenlocher -- Analysis of leukocyte migration through monolayers of cultured endothelial cells / Helen M. McGettrick, Lynn M. Butler, and Gerard B. Nash -- Biochemical purification of pseudopodia from migratory cells / Yingchung Wang and Richard L. Klemke -- Dynamic assessment of cell-matrix mechanical interactions in three-dimensional culture / W. Matthew Petroll -- Quantitative analyses of cell adhesion strength / Nathan D. Gallant and Andrés J. García -- Using RNA interference to knock down the adhesion protein TES / Elen Griffith -- Assaying calpain activity / Neil O. Carragher -- Analysis of focal adhesions and cytoskeleton by custom microarray / Matthew J. Dalby and Stephen J. Yarwood -- Proteomic analysis of cell surface membrane proteins in leukemic cells / Robert S. Boyd, Martin J.S. Dyer, and Kelvin Cain -- Bioinformatic analysis of adhesion proteins / Josephine C. Adams and Juergen Engel -- Analysis of integrin dynamics by fluorescence recovery after photobleaching / Bernhard Wehrle-Haller -- Double-hydrogel substrate as a model system for three-dimensional cell culture / Karen A. Beningo and Yu-li Wang -- In vitro actin assembly assays and purification from acanthamoeba / J. Bradley Zuchero. 
588 0 |a Print version record. 
520 |a The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion. 
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650 0 |a Cell adhesion.  |0 http://id.loc.gov/authorities/subjects/sh85021639 
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650 6 |a Molécules d'adhésion cellulaire. 
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