Yeast protocols /

Yeast protocols /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, N.J. : Humana Press, ©2006.
Description:	1 online resource (xi, 392 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; 313 Methods in molecular biology (Clifton, N.J.) ; 313.
Subject:	Yeast fungi -- Biotechnology -- Laboratory manuals. Yeast fungi -- Laboratory manuals. Yeasts -- physiology. Yeasts -- cytology. Yeasts -- isolation & purification. Yeast fungi. Yeast fungi -- Biotechnology. Laboratory Manual. Handbooks and manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11162493

Hidden Bibliographic Details
Other authors / contributors:	Xiao, Wei, 1957 April 10-
ISBN:	1588294374 9781588294371 9781592599585 1592599583 1280358815 9781280358814 9786610358816 6610358818 9781617375699 1617375691
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms.
Other form:	Print version: Yeast protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2006 1588294374
Standard no.:	10.1385/1592599583.

Description
Summary:	In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Physical Description:	1 online resource (xi, 392 pages) : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	1588294374 9781588294371 9781592599585 1592599583 1280358815 9781280358814 9786610358816 6610358818 9781617375699 1617375691