Calcium signaling protocols /

Calcium signaling protocols /

Saved in:
Bibliographic Details
Edition:2nd ed.
Imprint:Totowa, N.J. : Humana Press, ©2006.
Description:1 online resource (x, 359 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 312
Methods in molecular biology (Clifton, N.J.) ; volume 312.
Subject:
Calcium -- Laboratory manuals.
Calcium -- Research -- Methodology.
Calcium channels -- Research -- Methodology.
Cellular signal transduction -- Research -- Methodology.
Calcium -- pharmacokinetics.
Calcium Channels -- pharmacokinetics.
Calcium Signaling -- physiology.
Signal Transduction.
Spectrometry, Fluorescence -- methods.
Calcium.
Cellular signal transduction -- Research -- Methodology.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11162522
Hidden Bibliographic Details
Other authors / contributors:Lambert, David G.
ISBN:1588294420
9781588294425
9781592599493
1592599494
Notes:Includes bibliographical references and index.
Print version record.
Summary:Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition continues the excellence of the highly regarded first edition. New to this edition are chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for novice and expert researchers alike both theoretical and practical information for the optimal design and performance of studies of intracellular Ca2+ regulation
Other form:Print version: Calcium signaling protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2006 1588294420
Table of Contents:
  • Preface
  • Contributors
  • Part I. General
  • 1. Fluorescent Measurement of [Ca 2+ ] c : Basic Practical Considerations Alec
  • 2. Measurement of [Ca 2+ ] i in Whole Cell Suspensions Using Fura-2
  • 3. Measurement of [Ca 2+ ] i in Cell Suspensions Using Indo-1
  • Part II. Specialist Measurement Systems
  • 4. Confocal Microscopy: Theory and Applications for Cellular Signaling
  • 5. Single-Cell and Subcellular Measurement of Intracellular Ca 2+ Concentration
  • 6. Ratiometric Ca 2+ Measurements Using the FlexStation (r) Scanning Fluorometer
  • 7. Measuring Ca 2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
  • Part III. Measurement of Ca 2+ Channel Activity
  • 8. Measurement of Ca 2+ Entry Using 45 Ca 2+
  • 9. Measurement of [ 3 H]PN200-110 and [ 125 I]?-Conotoxin MVIIA Binding
  • 10. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca 2+ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons
  • Part IV. Measurement of ins(1,4,5)P 3 and Ca 2+ Release From Intracellular Stores
  • 11. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [ 3 H]Inositol-Labeling Protocols in Permeabilized Cells
  • 12. Measurement of Inositol(1,4,5)Triphosphate Using a Stereospecific Radioreceptor Mass Assay
  • 13. Measurement of Calcium Fluxes in Permeabilized Cells Using a 45 Ca 2+ Uptake and Release Assay
  • 14. Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells
  • 15. Measurement of Free [Ca 2+ ] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
  • Part V. Specialist Measurement Techniques
  • 16. Measurement of [Ca 2+ ] i in Smooth Muscle Strips Using Front-Surface Fluorimetry
  • 17. Measurement of Calcium and Movement in Heart Cells
  • 18. Simultaneous Analysis of Intracellular pH and Ca 2+ From Cell Populations
  • 19. Measurement of Cytosolic-Free Ca 2+ in Plant Tissue
  • Part VI. Ca 2+ -Sensitive Targets
  • 20. Assay and Purification of Calmodulin-Dependent Protein Kinase
  • 21. Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
  • 22. Measurement of Ca 2+ -ATPase Activity (in PMCA and SERCA1)
  • Index

Similar Items