Allergy methods and protocols /
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| Imprint: | Totowa, NJ : Humana Press, ©2008. |
|---|---|
| Description: | 1 online resource (xi, 352 pages) : illustrations |
| Language: | English |
| Series: | Methods in molecular medicine ; 138 Methods in molecular medicine ; 138. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11162725 |
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| 245 | 0 | 0 | |a Allergy methods and protocols / |c edited by Meinir G. Jones and Penny Lympany. |
| 260 | |a Totowa, NJ : |b Humana Press, |c ©2008. | ||
| 300 | |a 1 online resource (xi, 352 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file | ||
| 347 | |b PDF | ||
| 490 | 1 | |a Methods in molecular medicine ; |v 138 | |
| 588 | 0 | |a Print version record. | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |t Understanding of the molecular mechanisms of allergy / |r Meinir Jones -- |t T cell : primary culture from peripheral blood / |r Monika Raulf-Heimsoth -- |t Production of T-cell lines / |r Helga Kahlert -- |t Production of human T-cell clones / |r Adrienne Verhoef -- |t Mapping of human T-cell epitopes of allergens / |r Thomas Zeiler |g and |r Tuomas Virtanen -- |t Determining MHC restriction of T-cell responses / |r Mark Larché -- Short-term culture of CD8 cells and intracellular cytokine staining / |r Beejal Vyas |g and |r Alistair Noble -- |t Isolation, flow cytometric analysis, and suppression assay of CD4+ CD25+ T-regulatory cells / |r Hayley Jeal -- |t Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production / |r Sun-sang J. Sung -- |t Ultrasensitive ELISA for measurement of human cytokine responses in primary culture / |r William P. Stefura |g [and others] -- |t Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses / |r Monique Stinson |g [and others] -- |t Standardization of allergen extracts / |r Jørgen Nedergaard Larsen |g and |r Sten Dreborg -- |t Immunoelectrophoresis for the characterization of allergen extracts / |r Gitte Nordskov Hansen |g and |r Jørgen Nedergaard Larsen -- |t Conjugation of haptens / |r Ranulfo Lemus |g and |r Meryl H. Karol -- |t Monoclonal antibodies / |r Helga Kahlert |g and |r Oliver Cromwell -- |t Purification of antibodies / |r Per H. Larsson -- |t Collection of air samples to quantify exposure to airborne allergens / |r Susan Gordon -- |t Assay of air sample eluates / |r Anne Renström |g and |r Susan Gordon -- |t The halogen assay : a new technique for measuring airborne allergen / |r Euan Tovey |g [and others] -- |t Measurement of specific IgG anti-Fel d 1 antibodies / |r Meinir G. Jones -- |t The facilitated antigen binding (FAB) assay : a protocol to measure allergen-specific inhibitory antibody activity / |r James N. Francis -- |t Microscopic identification and purity determination of pollen grains / |r Magdalena Rahl -- |t Biopanning for the characterization of allergen mimotopes / |r Isabella Pali-Schöll |g and |r Erika Jensen-Jarolim -- |t Identification of mast cells and mast cell subpopulations / |r Mark Buckley |g and |r Andrew F. Walls -- |t Purification and characterization of mast cell tryptase and chymase from human tissues / |r Alan R. McEuen |g and |r Andrew F. Walls -- |t Experimental activation of mast cells and their pharmacological modulation / |r Shaoheng He |g and |r Andrew F. Walls -- |t In situ hybridization / |r Kayhan T. Nouri-Aria. |
| 520 | |a In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Protocols will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Allergy |v Laboratory manuals. | |
| 650 | 0 | |a Allergy. |0 http://id.loc.gov/authorities/subjects/sh85003662 | |
| 650 | 0 | |a Immunologic diseases. |0 http://id.loc.gov/authorities/subjects/sh85064571 | |
| 650 | 0 | |a Diseases. |0 http://id.loc.gov/authorities/subjects/sh85038411 | |
| 650 | 2 | |a Laboratory Manuals | |
| 650 | 2 | |a Hypersensitivity | |
| 650 | 2 | |a Immune System Diseases | |
| 650 | 2 | |a Publication Formats | |
| 650 | 2 | |a Disease | |
| 650 | 2 | |a Publication Characteristics | |
| 650 | 6 | |a Hypersensibilité (Immunologie) | |
| 650 | 6 | |a Maladies immunologiques. | |
| 650 | 6 | |a Maladies. | |
| 650 | 7 | |a Immunologic diseases |2 fast | |
| 650 | 7 | |a Diseases |2 fast | |
| 650 | 7 | |a Allergy |2 fast | |
| 653 | 0 | 0 | |a allergies |
| 653 | 0 | 0 | |a overgevoeligheid |
| 653 | 0 | 0 | |a hypersensitivity |
| 653 | 0 | 0 | |a immuniteitsreactie |
| 653 | 0 | 0 | |a immune response |
| 653 | 0 | 0 | |a allergenen |
| 653 | 0 | 0 | |a allergens |
| 653 | 0 | 0 | |a protocollen |
| 653 | 0 | 0 | |a protocols |
| 653 | 1 | 0 | |a Human Allergies |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft |0 http://id.loc.gov/authorities/genreForms/gf2014026120 | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf | |
| 700 | 1 | |a Jones, Meinir G. |0 http://id.loc.gov/authorities/names/nb2008010590 | |
| 700 | 1 | |a Lympany, Penny. |0 http://id.loc.gov/authorities/names/nb2008010592 | |
| 776 | 0 | 8 | |i Print version: |t Allergy methods and protocols. |d Totowa, N.J. : Humana Press, ©2008 |z 9780896038967 |w (DLC) 2007932488 |w (OCoLC)228168447 |
| 830 | 0 | |a Methods in molecular medicine ; |v 138. |x 1543-1894 |0 http://id.loc.gov/authorities/names/n88525800 | |
| 856 | 4 | 0 | |u https://link.springer.com/10.1007/978-1-59745-366-0 |y Springer Nature |
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