Allergy methods and protocols /

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Bibliographic Details
Imprint:	Totowa, NJ : Humana Press, ©2008.
Description:	1 online resource (xi, 352 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine ; 138 Methods in molecular medicine ; 138.
Subject:	Allergy -- Laboratory manuals. Allergy. Immunologic diseases. Diseases. Laboratory Manuals Hypersensitivity Immune System Diseases Publication Formats Disease Publication Characteristics Immunologic diseases Diseases Allergy Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11162725

Hidden Bibliographic Details
Other authors / contributors:	Jones, Meinir G. Lympany, Penny.
ISBN:	1597453668 9781597453660 0896038963 9780896038967
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Protocols will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy.
Other form:	Print version: Allergy methods and protocols. Totowa, N.J. : Humana Press, ©2008 9780896038967
Standard no.:	10.1007/978-1-59745-366-0

Table of Contents:

Understanding of the molecular mechanisms of allergy / Meinir Jones
T cell : primary culture from peripheral blood / Monika Raulf-Heimsoth
Production of T-cell lines / Helga Kahlert
Production of human T-cell clones / Adrienne Verhoef
Mapping of human T-cell epitopes of allergens / Thomas Zeiler and Tuomas Virtanen
Determining MHC restriction of T-cell responses / Mark Larché
Short-term culture of CD8 cells and intracellular cytokine staining / Beejal Vyas and Alistair Noble
Isolation, flow cytometric analysis, and suppression assay of CD4+ CD25+ T-regulatory cells / Hayley Jeal
Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production / Sun-sang J. Sung
Ultrasensitive ELISA for measurement of human cytokine responses in primary culture / William P. Stefura [and others]
Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses / Monique Stinson [and others]
Standardization of allergen extracts / Jørgen Nedergaard Larsen and Sten Dreborg
Immunoelectrophoresis for the characterization of allergen extracts / Gitte Nordskov Hansen and Jørgen Nedergaard Larsen
Conjugation of haptens / Ranulfo Lemus and Meryl H. Karol
Monoclonal antibodies / Helga Kahlert and Oliver Cromwell
Purification of antibodies / Per H. Larsson
Collection of air samples to quantify exposure to airborne allergens / Susan Gordon
Assay of air sample eluates / Anne Renström and Susan Gordon
The halogen assay : a new technique for measuring airborne allergen / Euan Tovey [and others]
Measurement of specific IgG anti-Fel d 1 antibodies / Meinir G. Jones
The facilitated antigen binding (FAB) assay : a protocol to measure allergen-specific inhibitory antibody activity / James N. Francis
Microscopic identification and purity determination of pollen grains / Magdalena Rahl
Biopanning for the characterization of allergen mimotopes / Isabella Pali-Schöll and Erika Jensen-Jarolim
Identification of mast cells and mast cell subpopulations / Mark Buckley and Andrew F. Walls
Purification and characterization of mast cell tryptase and chymase from human tissues / Alan R. McEuen and Andrew F. Walls
Experimental activation of mast cells and their pharmacological modulation / Shaoheng He and Andrew F. Walls
In situ hybridization / Kayhan T. Nouri-Aria.

Allergy methods and protocols /

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