Animal cell electroporation and electrofusion protocols /
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| Imprint: | Totowa, N.J. : Humana Press, ©1995. |
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| Description: | 1 online resource (xx, 369 pages) : illustrations |
| Language: | English |
| Series: | Methods in molecular biology ; v. 48 Methods in molecular biology (Clifton, N.J.) ; v. 48. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11163521 |
| Summary: | The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells. |
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| Physical Description: | 1 online resource (xx, 369 pages) : illustrations |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 1592595359 9781592595358 9781489940612 1489940618 1280836571 9781280836572 9786610836574 6610836574 089603304X 9780896033047 |