Antigen processing : methods and protocols /
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| Imprint: | New York : Humana Press, ©2013. |
|---|---|
| Description: | 1 online resource (xvii, 589 pages) : color illustrations |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; 960 Methods in molecular biology (Clifton, N.J.) ; 960. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11170146 |
MARC
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| 245 | 0 | 0 | |a Antigen processing : |b methods and protocols / |c edited by Peter van Endert. |
| 260 | |a New York : |b Humana Press, |c ©2013. | ||
| 300 | |a 1 online resource (xvii, 589 pages) : |b color illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 960 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |t Purification of large cytosolic proteases for in vitro assays: 20S and 26S proteasomes -- |t Analysis of proteasome generated antigenic peptides by mass spectrometry -- |t Using protease inhibitors in antigen presentation assays -- |t Kinetic studies of cytoplasmic antigen processing and production of MHC class I ligands -- |t Assaying peptide translocation by the peptide transporter TAP -- |t In vitro reconstitution of the MHC class I peptide-loading complex -- |t Studying mhc class i peptide loading and exchange in vitro -- |t Measuring synthesis and degradation of MHC class I molecules -- |t Studying ubiquitination of MHC class I molecules -- |t Evaluation of viral interference with MHC class I-restricted antigen processing and presentation using a flow cytometry-based approach -- |t Determining the activity of the transporter associated with antigen processing in the compartments of the secretory pathway -- |t Biochemical large-scale identification of MHC class I ligands -- |t Establishing MHC class I peptide motifs -- |t Quantitating MHC class I ligand production and presentation using TCR-like antibodies -- |t Biochemical analysis of naturally processed antigenic peptides presented by MHC class I molecules -- |t Identifying source proteins for MHC class I-presented peptides -- |t Purification, preparation, and use of chaperone-peptide complexes for tumor immunotherapy -- |t Recombinant poxviruses: Versatile tools for immunological assays -- |t Bioinformatics identification of antigenic peptide: Predicting the specificity of major MHC class I and II pathway players -- |t Evaluating CD8(+) T cell responses in vitro -- |t Cloning CD8(+) cytolytic T lymphocytes -- |t Production of CD4(+) and CD8 (+) T cell hybridomas -- |t Tracking antigen-specific CD8(+) T cells using MHC class I multimers -- |t The purification of large numbers of antigen presenting dendritic cells from mouse spleen -- |t Preparation of dendritic cells by in vitro cultures -- |t Monitoring dendritic cell activation and maturation -- |t Monitoring the intracellular routing of internalized antigens by immunofluorescence microscopy -- |t Isolation of a specialized, antigen-loaded early endosomal subpopulation by flow cytometry -- |t Preparing antigens suitable for cross-presentation assays in vitro and in vivo -- |t Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors -- |t Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading -- |t Assembly of matched [alpha/beta] subunits to HLA class II peptide receptors -- |t Studying MHC class II peptide loading and editing in vitro -- |t Study of the allogeneic response induced by endothelial cells expressing hla class ii after lentiviral transduction -- |t Antigen processing for mhc presentation via macroautophagy -- |t Studying MHC class II transport in dendritic cells -- |t In vitro digestion with proteases producing MHC class II ligands -- |t MHC-II ubiquitination -- |t Studying MHC class II presentation of immobilized antigen by B lymphocytes -- |t Production of primary human CD4(+) T cell lines and clones -- |t Analyzing antigen recognition by natural killer T cells -- |t Chromatofocusing purification of CD1B-antigen complexes and their analysis by isoelectric focusing. |
| 520 | |a Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing events, ending with recognition of peptide-MHC complexes at the cell surface by T lymphocytes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antigen Processing: Methods and Protocols is designed for beginners and experts interested in studying antigen processing. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Antigens |x Analysis |v Laboratory manuals. | |
| 650 | 2 | |a Antigen Presentation. | |
| 650 | 7 | |a Antigens |x Analysis. |2 fast |0 (OCoLC)fst00810524 | |
| 653 | 4 | |a Medicine. | |
| 653 | 4 | |a Immunology. | |
| 653 | 4 | |a Monoclonal antibodies. | |
| 653 | 4 | |a Biomedicine. | |
| 653 | 4 | |a Antibodies. | |
| 653 | 0 | 0 | |a biomedische wetenschappen |
| 653 | 0 | 0 | |a immunologie |
| 653 | 0 | 0 | |a antilichamen |
| 653 | 1 | 0 | |a Medicine (General) |
| 653 | 1 | 0 | |a Geneeskunde (algemeen) |
| 655 | 2 | |a Laboratory Manual. | |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 700 | 1 | |a Endert, Peter van. | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v 960. |x 1064-3745 | |
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| 928 | |t Library of Congress classification |a QR186.5 .A58 2013 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-62703-218-6 |z Springer Nature |g ebooks |i 12531810 | ||